The assessment of PFA-100 test for the estimation of blood loss in renal transplantation operation.

BACKGROUND

Platelet dysfunction is well known factor that may play a role in bleeding diathesis in uremia. In recent years, Platelet Function Assay 100 (PFA-100) was introduced to measure platelet function. The purpose of this study was to determine whether an abnormal PFA-100 is an accurate predictor of bleeding in dialysis patients undergoing renal transplantation (RTx).

MATERIAL/

METHODS

We included 98 dialysis patients undergoing RTx operation. PFA-100 test measuring collagen/epinephrine (Col/EPI) and collagen/adenosine 5'-diphosphate (Col/ADP) closure was performed in each patients after induction of anesthesia. We compared intraoperative blood loss measured by gravimetric method during RTx operation method between patients with normal Col/EPI and Col/ADP closure times (group 1, n= 51) and with prolonged Col/EPI and Col/ADP closure times (group 2, n=47).

RESULTS

Intraoperative blood loss calculated by gravimetric method was 273+/-50 ml in the group 1 and 303+/-109 ml in the group 2 (p>0.05). Blood loss in gross formula was 356+/-87 ml in the group 1 and 450+/-99 ml in group 2 (p>0.05).

CONCLUSIONS

Assessment of platelet function with preoperative measurement of PFA-100 in RTx patients is not an effective method for estimating the risk of blood loss in the intraoperative and postoperative periods.