Crystallographic studies of snake venom proteins from Taiwan cobra (Naja nana atra). Cardiotoxin-analogue III and phospholipase A2.

Orthorhombic crystals of cardiotoxin-analogue III from Taiwan cobra have been grown from 2-methyl-2,4-pentanediol solution. The space group is P222 with 2 molecules/asymmetric unit. Several different crystal forms of phospholipase A2 from Taiwan cobra have been produced with or without Ca2+ ion. One of them, grown from 30 mM sodium cacodylate buffer (pH 6 to 7) and 30% 2-methyl-2,4-pentanediol with 20 mg/ml of protein, diffracts well to 2 A resolution. It crystallizes in space group P3112 or P3212 with 2 molecules/asymmetric unit, but it can be related to a quasi-cubic space group P213.