Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2010
Dating of human bocavirus infection with protein-denaturing IgG-avidity assays-Secondary immune activations are ubiquitous in immunocompetent adults.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Hedman Lea, Söderlund-Venermo Maria, Jartti Tuomas, Ruuskanen Olli, Hedman Klaus
Аннотация
BACKGROUND: Human bocavirus (HBoV) is a widespread human parvovirus causing acute respiratory illness in young children. The HBoV primary infections are viremic and can be diagnosed serologically.
OBJECTIVES: To set up HBoV-IgG-avidity enzyme immuno assays (EIAs) using as antigen recombinant VP2 virus-like particles (VLPs), for diagnosis and timing of primary infections and their distinction from secondary infections or immunoactivations by this recently found virus.
STUDY DESIGN: The VLPs were utilized in setting up HBoV-IgG-avidity-EIAs of two different types. Paired sera were available from 36 wheezing children with acute primary HBoV infection, single sera from 108 nonsymptomatic university students, and 84 single or follow-up sera from 38 adults with pre-existing HBoV immunity.
RESULTS: HBoV-IgG avidity for the VP2-VLPs was measured successfully by protein-denaturing EIAs of two types, employing low concentrations of urea (4.7M and 2.5M). The diagnostic specificities were 99.1% and 90.7%, and diagnostic sensitivities, 94.4% and 91.7%, respectively. Interestingly, of the adults followed up 44% (4/9) exhibited significant titre increases of past-immunity HBoV-IgG.
CONCLUSIONS: Diagnosis of HBoV primary infection can be strengthened by measurement of IgG avidity. HBoV secondary infections or anamnestic antibody responses occur ubiquitously in immunocompetent adults.
OBJECTIVES: To set up HBoV-IgG-avidity enzyme immuno assays (EIAs) using as antigen recombinant VP2 virus-like particles (VLPs), for diagnosis and timing of primary infections and their distinction from secondary infections or immunoactivations by this recently found virus.
STUDY DESIGN: The VLPs were utilized in setting up HBoV-IgG-avidity-EIAs of two different types. Paired sera were available from 36 wheezing children with acute primary HBoV infection, single sera from 108 nonsymptomatic university students, and 84 single or follow-up sera from 38 adults with pre-existing HBoV immunity.
RESULTS: HBoV-IgG avidity for the VP2-VLPs was measured successfully by protein-denaturing EIAs of two types, employing low concentrations of urea (4.7M and 2.5M). The diagnostic specificities were 99.1% and 90.7%, and diagnostic sensitivities, 94.4% and 91.7%, respectively. Interestingly, of the adults followed up 44% (4/9) exhibited significant titre increases of past-immunity HBoV-IgG.
CONCLUSIONS: Diagnosis of HBoV primary infection can be strengthened by measurement of IgG avidity. HBoV secondary infections or anamnestic antibody responses occur ubiquitously in immunocompetent adults.