Analysis of platinum-based anticancer injections cisplatin and carboplatin in blood serum and urine of cancer patients by photometry, fluorometry, liquid chromatography using a Schiff-base as derivatizing reagent.

Photometric, fluorometric and liquid chromatographic methods were proposed to analyze Pt(II) from cis-platin and carbo-platin injections after derivatization with reagent 2-oxo-propanoic acid N-phenylhydrazonecarbothioamide. The reagent reacted with metals Au(III), Ag(I), Mn(II), Pt(II), Mo(VI), V(V/IV) to develop their characteristic colors in the pH range 3-12 and were extracted in organic solvent trichloromethane. The photoluminescent behavior of ligand and its metal complexes was investigated to correlate the emission pattern. Liquid chromatographic method was also proposed to analyze cis-platin and carbo-platin anti-cancer drugs based on the pre-column derivatizing platinum(II) with ligand. The complex of platinum was separated and eluted from HPLC column Microsorb C-18, (150 cm x 4.6 mm i.d, 5 µm) comprising eluents - tetrabutyl ammonium bromide (1 mM)-sodium acetate (1 mM)-acetonitrile-water-methanol (02:02:06:22:68 v/v/v/v/v). Metals Au(III), Ag(I), Mn(II), V(IV/V), Mo(VI) were also separated completely. The linear calibration range 0.5-2.5 µg/mL was observed following Beer's law with detection limit of 150.00 ng/mL Pt(II). The determination of cis-platin and carbo-platin injections by photometric, fluorometric and chromatographic methods showed RSD (n = 3) 1.14-3.12, 0.98-2.84, 0.92-2.72% respectively. The developed methods were employed to analyze cis-platin in samples of serum and urine of cancer patients undergoing chemotherapy and platinum amounts were observed within 45.0-86.0, 49.0-91.0, 42.0-84.0 ng/mL and 82.0-398, 81.0-389, 74.0-391 ng/mL with relative standard deviation (RSD) (n = 4) of 2.28-3.88, 2.40-3.82, 2.52-3.82% and 2.52-3.91, 2.44-3.94, 1.98-3.24% by liquid chromatographic, fluorometric and photometric techniques respectively.