[Protective effects of tanshinone IIA on injured primary cultured rat hepatocytes induced by CCl4].

OBJECTIVE

To investigate the effect of tanshinone IIA on carbon tetrachloridlde (CCl4) intoxicated primary cultured rat hepatocytes.

METHODS

The rat hepatocytes were separated by perfusion with collagenase solution. Tanshinone IIA was added to the cultured medium after incubating the rat hepatocytes for 36 hours. At the same time, CCl4 was added to induce hepatocytes injury for 1 h. The levels of ALT, LDH, NO, SOD, MDA and cell viability after cultivation for 3 h were determined respectively.

RESULT: Tanshinone IIA significantly improved cell viability, inhibited the decrease of SOD activity and the increase of ALT, LDH, MDA, NO levels

CONCLUSION

Tanshinone IIA protected effectively the primary cultured rat hepatocytes against CCl4-induced injury.