Fluids and barriers of the CNS · 2016
Immunohistochemical and in situ hybridization study of urate transporters GLUT9/URATv1, ABCG2, and URAT1 in the murine brain.
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Tomioka Naoko H, Tamura Yoshifuru, Takada Tappei, Shibata Shigeru, Suzuki Hiroshi, Uchida Shunya, Hosoyamada Makoto
Аннотация
BACKGROUND: Uric acid (UA) is known to exert neuroprotective effects in the brain. However, the mechanism of UA regulation in the brain is not well characterized. In our previous study, we described that the mouse urate transporter URAT1 is localized to the cilia and apical surface of ventricular ependymal cells. To further strengthen the hypothesis that UA is transported transcellularly at the ependymal cells, we aimed to assess the distribution of other UA transporters in the murine brain.
METHODS: Immunostaining and highly-sensitive in situ hybridization was used to assess the distribution of UA transporters: GLUT9/URATv1, ABCG2, and URAT1.
RESULTS: Immunostaining for GLUT9 was observed in ependymal cells, neurons, and brain capillaries. Immunostaining for ABCG2 was observed in the choroid plexus epithelium and brain capillaries, but not in ependymal cells. These results were validated by in situ hybridization.
CONCLUSIONS: We propose that given their specific expression patterns in ependymal, choroid plexus epithelial, and brain capillary endothelial cells in this study, UA may be transported by these UA transporters in the murine brain. This may provide a novel strategy for targeted neuroprotection.
METHODS: Immunostaining and highly-sensitive in situ hybridization was used to assess the distribution of UA transporters: GLUT9/URATv1, ABCG2, and URAT1.
RESULTS: Immunostaining for GLUT9 was observed in ependymal cells, neurons, and brain capillaries. Immunostaining for ABCG2 was observed in the choroid plexus epithelium and brain capillaries, but not in ependymal cells. These results were validated by in situ hybridization.
CONCLUSIONS: We propose that given their specific expression patterns in ependymal, choroid plexus epithelial, and brain capillary endothelial cells in this study, UA may be transported by these UA transporters in the murine brain. This may provide a novel strategy for targeted neuroprotection.
Предметные рубрики MeSH
ATP Binding Cassette Transporter, Subfamily G, Member 2Acetic AcidAnimalsBrainChloroformEpendymaEpitheliumFluorescent Antibody TechniqueGlucose Transport Proteins, FacilitativeIn Situ HybridizationMaleMethanolMiceMice, Inbred C57BLMice, KnockoutOrganic Anion TransportersRNA, MessengerTissue Fixation