Journal of ethnopharmacology · 2025
Acute toxicity study of ethanol extract of Potentilla freyniana Bornm rhizome in rats.
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Tian Tingting, Chen Yang, He Qin, Jiao Yangmiao, Chen Lingqun, Shu Maomao, Rehman Mujeeb Ur, Zhang Zaiqi, Cao Liang
Аннотация
ETHNOPHARMACOLOGY RELEVANCE: Rhizome of Potentilla freyniana Bornm, commonly known as "Ma Deng Ai" (MDA), is a widely used Dong ethnic medicine for the treatment of dysentery, malaria, bacterial infections, and traumatic bleeding.
AIMS OF THE STUDY: This study aims to evaluate the acute toxicity of MDA in vivo.
MATERIALS AND METHODS: In the acute toxicity study, MDA was administered to rats as a single oral dose of 1000 mg/kg, 2000 mg/kg, 4000 mg/kg, 8000 mg/kg, and 16000 mg/kg, along with a normal control group with distilled water in the same volume. Clinical signs of toxicity and general characteristics of animals were observed for 7 days. Water and food intake were recorded. After this hematological, biochemical, and histological analyses were performed.
RESULTS: The results of the experiments performed illustrated significant changes in the rat's weight, water intake, and food consumption in all MDA treated groups. At a dose of 4000 mg/kg, rats began to show mild symptoms of difficulty in breathing. At a dose of 8000 mg/kg, rats showed more severe symptoms of breathing difficulty, leading to mortalities in this group. At a dose of 16000 mg/kg, rats showed most severe symptoms of breathing difficulty, with restlessness, and died within 6 h of oral administration, demonstrating the Lethal Dose-50 (LD50) of 8510 mg/kg. The study indicates that the MDA treated groups showed significant changes in parts of hematological parameters such as mean corpuscle hemoglobin concentration (MCHC). The experiment groups have significant differences with control group in parameters of SGPT, SGOT, UREA, CRE and TG (p < 0.05∗). The histopathological analysis showed that at a dose of 16000 mg/kg, the rat's lung, liver, and kidneys showed pathological changes such as vascular congestion and dilation.
CONCLUSIONS: The present study demonstrated the significant changes caused by MDA such as histopathological changes in the lung, liver, and kidneys. Oral doses of MDA to 8000 and 16000 mg/kg in rats showed oral-related toxicity or death of animals. The death was related to lung injury. Histopathological examination displayed observable cellular damage in cell morphology, nuclear characteristics, and organ tissue integrity in lungs, liver and kidneys. While the groups with the dosage of 1000, 2000 and 4000 mg/kg showed significant change in the food and water intake pattern. These findings provide a strong rationale for advancing to in vivo (safety and efficacy) studies to explore the biological and pharmacological profile of MDA in greater detail.
AIMS OF THE STUDY: This study aims to evaluate the acute toxicity of MDA in vivo.
MATERIALS AND METHODS: In the acute toxicity study, MDA was administered to rats as a single oral dose of 1000 mg/kg, 2000 mg/kg, 4000 mg/kg, 8000 mg/kg, and 16000 mg/kg, along with a normal control group with distilled water in the same volume. Clinical signs of toxicity and general characteristics of animals were observed for 7 days. Water and food intake were recorded. After this hematological, biochemical, and histological analyses were performed.
RESULTS: The results of the experiments performed illustrated significant changes in the rat's weight, water intake, and food consumption in all MDA treated groups. At a dose of 4000 mg/kg, rats began to show mild symptoms of difficulty in breathing. At a dose of 8000 mg/kg, rats showed more severe symptoms of breathing difficulty, leading to mortalities in this group. At a dose of 16000 mg/kg, rats showed most severe symptoms of breathing difficulty, with restlessness, and died within 6 h of oral administration, demonstrating the Lethal Dose-50 (LD50) of 8510 mg/kg. The study indicates that the MDA treated groups showed significant changes in parts of hematological parameters such as mean corpuscle hemoglobin concentration (MCHC). The experiment groups have significant differences with control group in parameters of SGPT, SGOT, UREA, CRE and TG (p < 0.05∗). The histopathological analysis showed that at a dose of 16000 mg/kg, the rat's lung, liver, and kidneys showed pathological changes such as vascular congestion and dilation.
CONCLUSIONS: The present study demonstrated the significant changes caused by MDA such as histopathological changes in the lung, liver, and kidneys. Oral doses of MDA to 8000 and 16000 mg/kg in rats showed oral-related toxicity or death of animals. The death was related to lung injury. Histopathological examination displayed observable cellular damage in cell morphology, nuclear characteristics, and organ tissue integrity in lungs, liver and kidneys. While the groups with the dosage of 1000, 2000 and 4000 mg/kg showed significant change in the food and water intake pattern. These findings provide a strong rationale for advancing to in vivo (safety and efficacy) studies to explore the biological and pharmacological profile of MDA in greater detail.