Clinical hemorheology and microcirculation · 2001
Effects of acetylcholine and spermineNONOate on erythrocyte hemorheologic and oxygen carrying properties.
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Mesquita R, Pires I, Saldanha C, Martins-Silva J
Аннотация
PURPOSE OF THE STUDY: To determine the effects of acetylcholine and spermineNONOate--a NO donor--on RBC membrane and oxygen carrying properties.
MATERIAL AND METHODS: Aliquots of venous blood from eleven healthy subjects were incubated with ACh 10(-3) M, ACh 10(-5) M, spermineNONOate 10(-5) M and spermineNONOate 10(-4) M. The following parameters were determined: erythrocyte aggregation and deformability, hematocrit, plasma pH, osmolality, K(+), Na(+), Ca(2+) concentrations, hemoglobin, oxyhemoglobin, carboxyhemoglobin and methemoglobin concentrations, oxygen saturation of hemoglobin, oxygen and carbon dioxide partial pressures and p50.
RESULTS: In presence of ACh there is an increase of erythrocyte deformability, decrease of erythrocyte aggregation, plasma pH, K(+) and Na(+) concentration, increase of Ca(2+) concentration and p50. In presence of spermineNONOate there is an increase of erythrocyte deformability, plasma pH, decrease of Na(+) and Ca(2+) concentration, increase of metHb concentration and decrease of p50.
CONCLUSION: Acetylcholine and spermineNONOate are able to induce changes on RBC membrane and oxygen carrying properties.
MATERIAL AND METHODS: Aliquots of venous blood from eleven healthy subjects were incubated with ACh 10(-3) M, ACh 10(-5) M, spermineNONOate 10(-5) M and spermineNONOate 10(-4) M. The following parameters were determined: erythrocyte aggregation and deformability, hematocrit, plasma pH, osmolality, K(+), Na(+), Ca(2+) concentrations, hemoglobin, oxyhemoglobin, carboxyhemoglobin and methemoglobin concentrations, oxygen saturation of hemoglobin, oxygen and carbon dioxide partial pressures and p50.
RESULTS: In presence of ACh there is an increase of erythrocyte deformability, decrease of erythrocyte aggregation, plasma pH, K(+) and Na(+) concentration, increase of Ca(2+) concentration and p50. In presence of spermineNONOate there is an increase of erythrocyte deformability, plasma pH, decrease of Na(+) and Ca(2+) concentration, increase of metHb concentration and decrease of p50.
CONCLUSION: Acetylcholine and spermineNONOate are able to induce changes on RBC membrane and oxygen carrying properties.
Предметные рубрики MeSH
AcetylcholineAdultCarbon DioxideCarboxyhemoglobinCationsDose-Response Relationship, DrugErythrocyte AggregationErythrocyte DeformabilityErythrocyte MembraneErythrocytesHemoglobinsHemorheologyHumansHydrogen-Ion ConcentrationMaleMethemoglobinNitric OxideNitric Oxide DonorsNitrogen OxidesOsmolar ConcentrationOxygenOxyhemoglobinsPartial PressureSpermine