Journal of ethnopharmacology · 2026
Anticancer mechanisms of Poikilospermum suaveolens root fraction: ethnopharmacological relevance, signaling pathways, and in vitro/in silico studies on MCF-7 cells.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Mardiana Lia, Milanda Tiana, Hadisaputri Yuni Elsa, Chaerunisaa Anis Yohana, Puspadewi Ririn
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Poikilospermum suaveolens (Blume) Merr., traditionally used in Indonesian folk medicine, has been employed for its reputed anticancer properties. However, scientific evidence elucidating its molecular mechanisms against breast cancer remains limited.
AIM OF THE STUDY: This study aimed to evaluate the cytotoxic and molecular effects of the ethyl acetate root fraction of P. suaveolens (FEAPs) on MCF-7 breast cancer cells, focusing on apoptosis induction, signaling pathway modulation, and mechanistic insights through in vitro and in silico approaches.
MATERIALS & METHODS: Roots of P. suaveolens were collected, identified, and extracted. Ethyl acetate fractions were obtained by sequential solvent extraction. Phytochemical profiling was conducted using LC-HRMS/MS. Cytotoxicity was assessed by resazurin assay; antioxidant capacity by DPPH assay. Expression of malignancy, apoptosis, cell cycle, and autophagy-related genes was evaluated via RT-PCR. Morphological changes were observed microscopically. Molecular docking analyzed interactions between major compounds and Caspase-3 and Caspase-7.
RESULTS: FEAPs exhibited moderate cytotoxicity (IC50: 128.46 ± 0.75 ppm) against MCF-7 cells. LC-HRMS/MS revealed flavonoids, phenolics, and triterpenoids as dominant constituents. PCR showed downregulation of AKT, mTOR, and STAT-3, and upregulation of FADD, CASP-8, and CASP-9. Cyclin D1 and CDK-1 expression decreased, indicating cell cycle inhibition. Molecular docking revealed swertiajaponin exhibited strong affinity to Caspase-7.
CONCLUSION: FEAPs exerts anticancer effects in MCF-7 breast cancer cells by modulating multiple signalling pathways and likely promoting caspase mediated apoptosis, supporting its traditional use as an anticancer remedy.
AIM OF THE STUDY: This study aimed to evaluate the cytotoxic and molecular effects of the ethyl acetate root fraction of P. suaveolens (FEAPs) on MCF-7 breast cancer cells, focusing on apoptosis induction, signaling pathway modulation, and mechanistic insights through in vitro and in silico approaches.
MATERIALS & METHODS: Roots of P. suaveolens were collected, identified, and extracted. Ethyl acetate fractions were obtained by sequential solvent extraction. Phytochemical profiling was conducted using LC-HRMS/MS. Cytotoxicity was assessed by resazurin assay; antioxidant capacity by DPPH assay. Expression of malignancy, apoptosis, cell cycle, and autophagy-related genes was evaluated via RT-PCR. Morphological changes were observed microscopically. Molecular docking analyzed interactions between major compounds and Caspase-3 and Caspase-7.
RESULTS: FEAPs exhibited moderate cytotoxicity (IC50: 128.46 ± 0.75 ppm) against MCF-7 cells. LC-HRMS/MS revealed flavonoids, phenolics, and triterpenoids as dominant constituents. PCR showed downregulation of AKT, mTOR, and STAT-3, and upregulation of FADD, CASP-8, and CASP-9. Cyclin D1 and CDK-1 expression decreased, indicating cell cycle inhibition. Molecular docking revealed swertiajaponin exhibited strong affinity to Caspase-7.
CONCLUSION: FEAPs exerts anticancer effects in MCF-7 breast cancer cells by modulating multiple signalling pathways and likely promoting caspase mediated apoptosis, supporting its traditional use as an anticancer remedy.