Journal of agricultural and food chemistry · 2016
Isolation and Identification of an Antiproliferative Compound from Fructose-Tryptophan Maillard Reaction Products.
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Lee Sang Hoon, Jeong Su Jeong, Jang Gwi Yeong, Kim Min Young, Hwang In Guk, Kim Hyun Young, Woo Koan Sik, Hwang Bang Yeon, Song Jin, Lee Junsoo, Jeong Heon Sang
Аннотация
This study was performed to isolate and identify a compound with antiproliferative activity against human stomach cancer cell lines, from fructose-tryptophan Maillard reaction products (MRPs). The MRPs, prepared from a fructose-tryptophan solution heated at 130 °C for 2 h, were fractionated into five solvent fractions: n-hexane, chloroform, ethyl acetate, butanol, and water. The highest antiproliferative activity was found in the chloroform fraction (85.93% at 200 μg/mL), and the active compound from this chloroform fraction was purified by silica gel column chromatography, TLC, and preparative HPLC. The antiproliferative activity (IC50) of the active compound was 42.24 μg/mL, and the active compound was identified as perlolyrine (C16H10N2O2) by (1)H/(13)C NMR, DEPT, HMBC, and LC-ESI-MS. Therefore, this research may be useful in developing perlolyrine as a functional therapeutic agent.