Evaluation of Antinociceptive and Anti-inflammatory Activity of Phytosterol Present in Chloroform Extract of Phyllanthus Maderaspatensis.

Inflammation and pain is a common cause of medical consultation which generally occurs when a tissue is injured. In recent scenario, attention has been paid for screening new drugs with analgesic activity from plant sources, to reduce or treat pain with less adverse effects than allopathic drugs. The objectives of this research work was to evaluate the antinociceptive and anti-inflammatory activity of phytosterol present in chloroform extract of Phyllanthus maderaspatensis (CEPM) through carrageenan-induced hind paw oedema and hot plate method in male wistar rats. The test groups received CEPM (suspended in 1% caboxymethylcellulose, CMC) at doses 400 mg/kg, p.o.; the reference group received indomethacin (10 mg/ kg, in 1% CMC, p.o.) as positive control and the negative control animals received the vehicle only (1% CMC, 10 mL/kg, p.o.). Oral administration of CEPM or indomethacin showed significant (P<0.001) effect in the reduction of the paw oedema induced by 0.1 mL of 1% carrageenan (in 1% CMC) solution into the subplantar region. CEPM showed the highest inhibition 71.42% at 3rd hr. CEPM extract and pentazocin significantly (P<0.001) increases the basal reaction time when compare to control group. The present study demonstrates the potential anti-inflammatory and analgesic effect of the CEPM which supports the claims by the traditional medicine practitioners.