3D tracking of ferrous iron in the epileptic mouse brain and screening of iron homeostasis regulators with a two-photon fluorescent probe.

The development of techniques for tracking active ferrous iron (Fe2+) distribution has greatly promoted the biological studies of iron. Here, we present an innovative application of a 3D two-photon fluorescent probe for Fe2+ tracking in the epileptic mouse brain, which has expanded the toolbox of screening for iron homeostasis regulators and contributed to the discovery of new chemical entities for the treatment of epilepsy. For complete details on the use and execution of this protocol, please refer to Shao et al. (2022).