[Effect and mechanism of three kinds of drinking water on blood pressure of hypertensive mice].

OBJECTIVE

To explore the effect of three kinds of drinking water and its possible mechanism on blood pressure of hypertensive mice.

METHODS

The water quality parameters were measured for three kinds of drinking water, and the parameters includedtotal dissolved solids( TDS), oxygen consumed( OC), p H, oxidation reduction potential( ORP), electric conductivity( EC), dissolved hydrogen, calcium and magnesium. Establishment of mouse model of high blood pressure by using N'-nitro-L-arginine methyl ester hydrochloride( L-NAME) for 30 days. Then the mice were randomly divided into 4 groups: normal control group, model control group, group of tap water and group of filtered water. Blood pressure in mice was detected every month. After 3 months, nitric oxide( NO), nitric oxide synthase( NOS), total superoxide dismutase( T-SOD), malondialdehyde( MDA), glutathione peroxidase( GSH-Px), endothelin( ET), angiotensin( Ang), aldosterone( ALD), catecholamine( CA), high-sensitivity C-reactive protein( hs-CRP), interleukin 6( IL-6) and cyclic guanosine monophosphate( c-GMP) in serum of the mice were determined. Simultaneously morphological changes of heart, kidney and thoracic aorta paraffin section were observed.

RESULTS

The p H, TDS, OC, EC, calcium and magnesium in filtered water were higher than that of pure and tap water. The negative ORP and great quantities of dissolved hydrogen were found in filtered water; The systolic blood pressure and mean blood pressure(( 106. 24 ± 5. 31) and( 90. 73 ± 4. 99)mm Hg) of filtered water were lower than those of the pure water( 119. 58 ± 6. 08 and 96. 44± 6. 48 mm Hg)( P < 0. 05). The NO(( 87. 05 ± 39. 82) μmol/L) in the mice with filtered water were higher than those of with pure and tap water(( 45. 01 ± 9. 62) and( 46. 56 ±30. 54) μmol/L)( P < 0. 05). Compared with those of the mice with pure water, significant decrease in IL-6(( 201. 42 ± 36. 41) and( 173. 99 ± 114. 96) vs. ( 363. 14 ± 149. 00)pg/m L) and Ang(( 1319. 20 ± 111. 90) and( 1349. 38 ± 180. 15) vs. ( 1736. 17 ±242. 86) ng/L) were observed in mice treated with tap and filtered water( P < 0. 05). The T-SOD and GSH-Px(( 268. 37 ± 12. 25) and( 712. 45 ± 30. 59) U/m L) in the mice with filtered water were significant higher than those of pure water(( 250. 46 ± 15. 60) and( 678. 36 ± 35. 80) U/m L)( P < 0. 05).

CONCLUSION

Compared with pure and tap water, filtered water can reduce the blood pressure of the hypertensive mice by antioxidation and anti-inflammatory.