Fa yi xue za zhi · 2017
[Determination of Sulfide Ion in Blood from Hydrogen Sulfide Poisoning Cases].
СтатьяНазвание и аннотация: Перевод в очереди
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Qiang H S, Chen H, Shen B H, Shen M, Xiang P
Аннотация
OBJECTIVES: To establish a gas chromatography-mass spectrometry （GC-MS） method for the determination of sulfide ion in blood and apply it to the practical cases.
METHODS: The 1, 3, 5-tribromobenzene was selected as an internal standard, and 0.2 mL blood sample was collected and analyzed using GC-MS after α-Bromo-2, 3, 4, 5, 6-pentafluorobenzyl bromide derivatization.
RESULTS: The mass concentration of sulfide ion in blood had good linearity in the range of 0.2-40 μg/mL with a limit of detection （LOD） of 0.05 μg/mL. The mass concentration of sulfide ion was less than 0.05 μg/mL in blank blood from different sources such as healthy subjects and dead cases. In 3 sulfide poisoning cases, sulfide ion was detected in the blood samples of 6 victims, and the mass concentration range was 1.02-3.13 μg/mL.
CONCLUSIONS: This study establishes a method for investigation of sulfide ion in blood which has been applied successfully to the cases of fatal sulfide poisonings.
METHODS: The 1, 3, 5-tribromobenzene was selected as an internal standard, and 0.2 mL blood sample was collected and analyzed using GC-MS after α-Bromo-2, 3, 4, 5, 6-pentafluorobenzyl bromide derivatization.
RESULTS: The mass concentration of sulfide ion in blood had good linearity in the range of 0.2-40 μg/mL with a limit of detection （LOD） of 0.05 μg/mL. The mass concentration of sulfide ion was less than 0.05 μg/mL in blank blood from different sources such as healthy subjects and dead cases. In 3 sulfide poisoning cases, sulfide ion was detected in the blood samples of 6 victims, and the mass concentration range was 1.02-3.13 μg/mL.
CONCLUSIONS: This study establishes a method for investigation of sulfide ion in blood which has been applied successfully to the cases of fatal sulfide poisonings.