[Immunocytochemical study of mononuclear cells in peripheral blood and cerebrospinal fluid of patients with Japanese B encephalitis].

Using the technique of monoclonal antibody-immunogold-silver staining (M-IGSS), the mononuclear cells in peripheral blood (PB) and cerebrospinal fluid (CSF) were screened for immunoreactivity against JBE virus antigen (JBEV-Ag), interleukin-2 receptor (Tac), class II human leucocyte antigen (HLA-DR) and interferon-r (IFN-r) in 17 ELISA diagnosed JEB patients and 17 control cases with non-inflammatory and non-immunological diseases. It was found that JBEV-Ag+ cells were present in PB (16/17) and CSF (12/17) of the JBE group, but absent in both the control group and another 5 cases with mumps virus meningitis. The percentages of Tac+, HLA-DR+ and IFN-r+ cells in JBE patients were higher than those in controls.