[Adenovirus-mediated interleukin-24 enhances the inhibitory effect of paclitaxel on the growth of lung cancer A549 cells].

OBJECTIVE

To investigate the effect of adenovirus-mediated interleukin-24 (Ad-IL-24) combined with paclitaxel on the growth of human lung cancer A549 cells in vitro.

METHODS

A549 cells were treated with Ad-IL-24 alone, paclitaxel alone and Ad-IL-24 combined with paclitaxel, respectively. Their proliferation was detected by CCK-8 assay, and the changes of apoptosis and cell cycle were tested by flow cytometry.

RESULTS

The treatment of Ad-IL-24 significantly inhibited the A549 cell growth and induced cell apoptosis. Compared with paclitaxel alone and Ad-IL-24 alone, the Ad-IL-24 plus paclitaxel treatment more evidently inhibited lung cancer cell growth and increased cell apoptosis rate, and induced G2/M phase arrest.

CONCLUSION

Ad-IL-24 infection can enhance the inhibitory effect of paclitaxel on lung cancer cell growth.