Proangiogenic effect and molecular mechanism of a new cytosporin derivative from the marine fungus Eutypella sp. D-1.

A new cytosporin derivative, cytoeutypellane D, was isolated from a culture extract of the Arctic-derived fungus Eutypella sp. D-1. Its structure was determined based on a detailed nuclear magnetic resonance spectroscopic interpretation, a mass spectrometry data analysis, and a literature review. Zebrafish were used in in vivo experiments to discover that the isolated compound significantly promoted angiogenesis in a dose-dependent manner. Further studies regarding the underlying mechanisms revealed that it may target the VEGF/VEGFR2 signaling pathway and rescue abnormal expressions of the cdk1, aurkb, topbp1, and brca2 genes to improve vascular endothelial cell proliferation and migration in zebrafish. In summary, our findings suggested that this novel compound isolated from the fungus Eutypella sp. D-1 might be used as a promising molecule for vascular injury disease treatment.