[Anti-inflammatory constituents in Urtica dioica subsp. gansuensis, a medical plant endemic to China].

The chemical constituents in Urtica dioica subsp. gansuensis were isolated systematically and identified by preparative high performance liquid chromatography(HPLC), nuclear magnetic resonance(NMR), high-resolution mass spectrometry(HRMS), and other methods. Their anti-inflammatory activities were evaluated in vitro. As a result, 33 compounds were isolated and identified as(7R,8S,8'R)-olivil-4-O-β-D-glucopyranoside(1), oleanic acid(2), vanillic acid(3), L-ascorbic acid(4), p-hydroxybenzaldehyde(5), rosmarinic acid(6), p-hydroxybenzoic acid(7), salicylic acid(8), ficusal(9), lumichrome(10), lobechine(11), 2-[formyl-5-(butyl ether methyl)-1H-pyrrol-1-yl] butyric acid butyl ester(12), 2-[formyl-5-(methoxymethyl)-1H-pyrrol-1-yl] butyric acid methyl ester(13), 2-[formyl-5-(methoxymethyl)-1H-pyrrol-1-yl] butyric acid butyl ester(14), benzoic acid(15), roseoside(16), benzyl-O-β-D-glucopyranoside(17), arbutin(18), carvone(19), trans-pinocarveol(20), carvacrol(21), taxifolin(22), pinocembrin(23), isovitexin(24), 3,4-divanillyltetrahydrofuran(25), apigenin-7-O-β-D-glucuronide(26), apigenin(27), β-ionone(28), trans-anethole(29), apigenin-7-O-β-D-glucopyranoside(30), afzelin(31), luteolin(32), and dehydrovomifoliol(33). Compound 1 was a new compound. Various phenolic acids(6-8, and 18) and alkaloids(11-14) exhibited significant anti-inflammatory activities and could significantly inhibit lipopolysaccharide(LPS)-induced NO release from RAW264.7 cells.