Wiadomosci lekarskie (Warsaw, Poland : 1960) · 2023
AGE AND GENDER-SPECIFIC FEATURES OF CYTOGENETIC CHANGES IN BUCCAL EPITHELIUM IN INDIVIDUALS RESIDING IN THE «SICK BUILDING».
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Oboronova Tetiana S, Kurdil Nataliia V, Palamar Borys I, Lisovska Viktoriia S, Khomenko Iryna M, Zhminko Petro G, Ustinova Lyudmila A
Аннотация
OBJECTIVE: The aim: The study of cytomorphological and cytogenetic features of the buccal epithelium of residents of apartments who complained of unpleasant odors in their homes.
PATIENTS AND METHODS: Materials and methods: The state of buccal epithelium in residents of multi-story buildings was studied. A total of 237 individuals were examined, 117 males and 120 females, aged from 6 to 81 years. Buccal cells were collected using a sterile spatula and stained with a 2.5% solutionofaceto-orcein and 1% light green. The preparations were examined using a light microscope OPTON Axioskop (Germany) with oil immersion at a magnification of x1000. Statistical processing of the data was performed using IBMSPSS Statistics 29.0.0.0 (t-Student criterion; Mann-Whitney; ANOVA: Tukey; T3-Dunnett), with p≤0.05.
RESULTS: Results: Cytomorphological and cytogenetic abnormalities, compared to physiological limits, were mainly manifested as karyorrhexis, nuclear doubling, the appearance of epitheliocytes with perinuclear vacuoles, or nuclear vacuolization. The frequency of micronuclei was observed in the range of (0.3-2.8 ‰). The highest micronucleus index (per 1000 cells, ‰) was observed among males aged 15-39 years and females over 65 years old. In both sexes, the lowest micronucleus indices were found in the age group of 6-14 years.
CONCLUSION: Conclusions: in the «sick building» an increase in the frequency of micronucleus occurrence among males and females was observed simultaneously with increasing age.
PATIENTS AND METHODS: Materials and methods: The state of buccal epithelium in residents of multi-story buildings was studied. A total of 237 individuals were examined, 117 males and 120 females, aged from 6 to 81 years. Buccal cells were collected using a sterile spatula and stained with a 2.5% solutionofaceto-orcein and 1% light green. The preparations were examined using a light microscope OPTON Axioskop (Germany) with oil immersion at a magnification of x1000. Statistical processing of the data was performed using IBMSPSS Statistics 29.0.0.0 (t-Student criterion; Mann-Whitney; ANOVA: Tukey; T3-Dunnett), with p≤0.05.
RESULTS: Results: Cytomorphological and cytogenetic abnormalities, compared to physiological limits, were mainly manifested as karyorrhexis, nuclear doubling, the appearance of epitheliocytes with perinuclear vacuoles, or nuclear vacuolization. The frequency of micronuclei was observed in the range of (0.3-2.8 ‰). The highest micronucleus index (per 1000 cells, ‰) was observed among males aged 15-39 years and females over 65 years old. In both sexes, the lowest micronucleus indices were found in the age group of 6-14 years.
CONCLUSION: Conclusions: in the «sick building» an increase in the frequency of micronucleus occurrence among males and females was observed simultaneously with increasing age.