Synthesis of novel liquid phase exfoliation of chitosan/Bi2Se3 hybrid nanocomposites for in-vitro wound healing.

Numerous studies have recently established the potential of chitosan (Chi) to enhance wound healing. Chi is a carbohydrate biopolymer that is biocompatible, low-cost, toxic-free, and has excellent antibacterial properties. In this study, we synthesized Chi/Bi2Se3 hybrid nanocomposites (NCs) using a liquid exfoliation approach. The physicochemical characterization of the hybrid NCs was investigated using X-ray diffraction, Fourier transforms infrared, Thermogravimetric, Scanning electron microscope, and Transmission electron microscope. The antibacterial ability has been investigated versus two pathogens, S. aureus and E. coli. In comparison to bare materials, the hybrid NCs demonstrated better antibacterial activity against both bacterial strains. As a result, the electrostatic attraction of positively charged Chi can easily attract the negatively charged surface of the bacteria cell membrane and NCs generate reactive oxygen species (ROS). This ROS can attack bacteria's intracellular components and eventually kill bacteria. The biocompatibility of the Chi/Bi2Se3 NCs was evaluated against L929 mice fibroblast cells, and there was no evident cytotoxicity. Furthermore, an in-vitro wound scratch test was carried out on L929 mouse fibroblast cells and the Chi/Bi2Se3 hybrid NCs promote wound healing and cell proliferation. These findings suggest that the Chi/Bi2Se3 hybrid NCs as a promising future material for bacteria-infected in-vivo wound healing.