Fish scale gelatin/diatom biosilica composite hemostasis sponge with ultrafast dispersing and in situ gelation for hemorrhage control.

Rapid control of hemorrhage is vital in first-aid and surgery. As representative of emergency hemostatic materials, inorganic porous materials achieve rapid hemostasis through concentrating protein coagulation factors by water adsorption to accelerate the coagulation reaction process, however their efficacy is often limited by the insufficient contact of material with blood and the lack of blood clot strength. Herein, we report an ultrafast dispersing and in situ gelation sponge (SG/DB) based on anchoring interface effect for hemorrhage control using freeze drying method after mixing fish scale gel (SG) and tert-butyl alcohol (TBA) pre-crystallized diatom biosilica (DB). This design retains the hierarchical porous structure of DB in SG matrix, and granting the SG/DB the capability to disperse ultrafast, achieving dissolution in both water and blood within 3 s. The DB and SG released by disintegration of SG/DB can activate intrinsic coagulation pathway and strengthen fibrin clot gelation through the anchoring interface effect, even realizing coagulation of anticoagulant whole blood without calcium ion activation. Animal studies showed 10%T-SG/DB has superior hemostatic properties to various commercially available hemostatic materials (rat liver and artery, 100 s; rabbit liver, artery, and heart, 3.2, 4.6, and 2.9 min, respectively), reducing bleeding by 30 % compared to QuikClot Combat Gauze®, and is easily removable without residue.