EFFECT OF TOBACCO SMOKE ON ROS PRODUCTION AND INFLAMMATION IN RATS OF DIFFERENT AGE.

The aim of our study was to investigate the effect of tobacco smoke on the content of reactive oxygen species (ROS) and level of cytokines in blood of rats of different age groups. For the purpose of carrying out the dosage, the beards of the white mongrel male rats were sub-divided into three groups: immature, mature and senile rats. Rat experimental groups were exposed to tobacco smoke daily for 45 days. The model of dependence on the chronic action of tobacco smoke was created using a sealed chamber in volume of 30 liters, which allowed to immerse animals in free behavior. Blood and blood serum was used for research. The isolation of neutrophils and lymphocytes from the blood was carried out by the method of gradient centrifugation on the double gradient of density ficol-verografin. The ROS production level was analyzed according to the intensity of the fluorescence. The immune enzyme method, using test systems in the blood serum, determined the level of proinflammatory cytokine, interleukin 6 (IL-6) and anti-inflammatory - interleukin 4 (IL-4). It was found that exposure to tobacco smoke in rats developed oxidative stress, as evidenced by increased formation of ROS in neutrophils and blood lymphocytes of all the study groups of animals. In this case, the level of ROS depends not only on the age of the animals, but also on the duration of the action of tobacco smoke. In the blood serum of all age groups of rats, pronounced changes in IL-6 content were observed on the 45th day of exposure to tobacco smoke. The highest IL-6 content was observed in immature rats at the end of the experiment. . At the same time, the content of anti-inflammatory cytokine IL-4 was significantly decreased in all age groups of animals. The results indicate an imbalance between the level of pro- and anti-inflammatory cytokines, in particular IL-6 and IL-4. The result of exposure to tobacco smoke is the development of oxidative stress and inflammatory processes that depend on the duration of the smoke, its concentration, the age of the animals, and other biologically active constituents.