Characterization and preparation of Fe3O4 nanoparticles loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment.

In this study, bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating was developed to produce bioactive and antibacterial coatings. The nanocomposite coating was embedded in chitosan (CS) matrix and coating was fabricated by electrophoretic deposition (EPD) method. The coating was characterized by using SEM/EDX and XRD respectively. The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating. The antibacterial activity was examined in Escherichia coli and Staphylococcus aureus bacteria which determine that the growth of microorganisms was inhibited with the progressive increment of Fe3O4 nanoparticles. The bioactivity assessment was done in PBS for 7 days and it was detected that the composite coatings improve the bone-bonding ability which was again confirmed by SEM-EDX. The corrosion behavior was evaluated in Ringer's solution by electrochemical test. The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings. The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.