[Use of kinetics of Fe+2-induced chemiluminescence in a tris-buffer liposome suspension for studying the antioxidant activity of blood plasma].

In order to evaluate antioxidant activity (AOA) of different preparations in respect to Fe(2+)-induced lipid peroxidation (LPO) chemiluminescence (CL) kinetics in tris-buffer liposome suspension was proposed. For studying AOA stages of fast flash, latent period and slow flash related to LPO reaction development were used. The measurement of CL kinetic parameters along with malondialdehyde accumulation makes it possible to determine also the mechanism of antioxidant effect on the preparations under consideration. The mechanism of AOA of the blood plasma is mainly due to transferrin, ceruloplasmin and HCO3- interaction with Fe2+ leading to abstraction of some part of ferrous ions participated in LPO reactions. In the case of albumin the change of CL kinetic parameters was proposed to be conditioned by the competition with ferrous ions for binding sites on liposomes. The effect of a-tocopherol at physiological concentrations compared with other blood plasma components in this system was negligibly small.