PLoS neglected tropical diseases · 2025
Trypanosoma cruzi (Chagas, 1909) transmission among captive wild mammals, triatomines and free-living opossums from surrounding areas in the São Paulo Zoological Park, Brazil.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Ferreira Suelen Sanches, Bueno Marina Galvão, Nery Carolina V C, Rassi Fabrício B, Monticelli Cauê, Chagas Carolina R F, Gonzalez Irys H L, das Chagas Xavier Samanta Cristina, Lisboa Cristiane Varella, Ramos Patrícia Locosque, Roque André Luiz Rodrigues
Аннотация
BACKGROUND: Trypanosoma cruzi is a multi-host parasite that infects dozens of mammalian species in the most variable wild environments in Americas. Between 2013 and 2018, autochthonous infections by T. cruzi were suspected in three European wolves (Canis lupus) and an orange dwarf porcupine (Coendou spinosus) from Fundação Parque Zoológico de São Paulo (FPZSP), Brazil, current Coordenadoria de Fauna Silvestre (CFS), which is inserted in a remnant of the Atlantic rainforest inside one of the biggest and most populous municipality of the world. This study aims to detect T. cruzi infections in captive mammals, triatomines and free-living opossums from surrounding areas of FPZSP/CFS.
METHODOLOGY/PRINCIPAL FINDINGS: Blood samples from captive and free-living mammals from surrounding areas were collected for parasitological (direct examination and culture), serological (IFAT) and molecular diagnosis using Nested-PCR 18SrDNA followed by DNA sequence analysis. Triatomines (Panstrongylus megistus) found in FPZSP/CFS were also examined by culture of the digestive tract and PCR. Trypanosoma cruzi infection was detected in 35.7% (n = 60/168 - 106 captive and 62 free-living) of the mammals that belonged to nine different families. From captive mammals, positive T. cruzi serology was observed in 29.6% (n = 27/91). Twenty-six positive hemocultures were obtained, from which parasite isolation was achieved in 69.2%, while positive PCR was observed in 40% of them, including nine free-living individuals that were also positive in hemoculture. Of 28 individuals in which T. cruzi characterization was successful, 89.3% were genotyped as DTU TcI, 7.2% as TcII and 3.5% as TcI/TcII mixed infection. Besides, 29 of 30 collected triatomines were infected, and infection by T. cruzi DTU TcI was confirmed in 16 of them.
CONCLUSIONS/SIGNIFICANCE: The confirmed autochthonicity of at least 68.9% of cases demonstrates that captive mammals from FPZSP are immersed in the T. cruzi enzootic cycle that involves the vector species P. megistus, and the reservoir hosts C. spinosus and Didelphis aurita from the wild surrounding areas.
METHODOLOGY/PRINCIPAL FINDINGS: Blood samples from captive and free-living mammals from surrounding areas were collected for parasitological (direct examination and culture), serological (IFAT) and molecular diagnosis using Nested-PCR 18SrDNA followed by DNA sequence analysis. Triatomines (Panstrongylus megistus) found in FPZSP/CFS were also examined by culture of the digestive tract and PCR. Trypanosoma cruzi infection was detected in 35.7% (n = 60/168 - 106 captive and 62 free-living) of the mammals that belonged to nine different families. From captive mammals, positive T. cruzi serology was observed in 29.6% (n = 27/91). Twenty-six positive hemocultures were obtained, from which parasite isolation was achieved in 69.2%, while positive PCR was observed in 40% of them, including nine free-living individuals that were also positive in hemoculture. Of 28 individuals in which T. cruzi characterization was successful, 89.3% were genotyped as DTU TcI, 7.2% as TcII and 3.5% as TcI/TcII mixed infection. Besides, 29 of 30 collected triatomines were infected, and infection by T. cruzi DTU TcI was confirmed in 16 of them.
CONCLUSIONS/SIGNIFICANCE: The confirmed autochthonicity of at least 68.9% of cases demonstrates that captive mammals from FPZSP are immersed in the T. cruzi enzootic cycle that involves the vector species P. megistus, and the reservoir hosts C. spinosus and Didelphis aurita from the wild surrounding areas.