CEC separation of aromatic compounds and proteins on hexylamine-functionalized N-acryloxysuccinimide monoliths.

Reactive organic polymer monoliths were prepared in fused-silica capillaries by UV-initiated free radical polymerization of N-acryloxysuccinimide (NAS) as reactive monomer, ethylene dimethacrylate as crosslinker, azobisisobutyronitrile as initiator, and toluene as porogen. In a second synthetic step, chemical derivatization of the activated-ester moieties was performed in situ through alkylation reaction with alkylamines to afford monolithic stationary phases with potential reversed-phase properties. A correlation between the synthesis conditions--composition of the reactive solution--chemical characteristics of the reactive polymer monoliths--nitrogen/NAS content--and the reversed-phase separation properties of the functionalized monolithic columns--selectivity towards homologous series of akylbenzenes--was clearly established. This finding offers the possibility of adjusting the experimental conditions with respect to the target applications. The monolithic stationary phases with optimized chemical and porous structures were used for the CEC separation of alkylbenzenes, phenols, anilines, organic acids, amino acids, and proteins. The data indicate that depending on the nature of the analytes (charge, hydrophobic/hydrophilic balance, size) reversed-phase or mixed modes may account for the observed separation.