Clinical cancer research : an official journal of the American Association for Cancer Research · 2013
DNA demethylating agents synergize with oncolytic HSV1 against malignant gliomas.
СтатьяПоддержка NIH: внешняя программаНазвание и аннотация: Перевод в очереди
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Okemoto Kazuo, Kasai Kazue, Wagner Benjamin, Haseley Amy, Meisen Hans, Bolyard Chelsea, Mo Xiaokui, Wehr Allison, Lehman Amy, Fernandez Soledad, Kaur Balveen, Chiocca E Antonio
Аннотация
PURPOSE: Oncolytic viruses (OV) based on herpes simplex virus type 1 (HSV1) are being used in clinical trials for a variety of cancers. The OV, rQNestin34.5, uses a nestin promoter/enhancer to selectively drive robust viral replication in malignant glioma cells. We have discovered that this promoter becomes extensively methylated in infected glioma cells, reducing OV efficacy.
EXPERIMENTAL DESIGN: We used demethylating drugs [5-azacytidine (5-Aza)], decitabine, or valproic acid (VPA) in both in vitro and in vivo malignant glioma models to determine if they improved the efficacy of rQNestin34.5 therapy.
RESULTS: The use of demethylating agents, such as 5-Aza, improved OV replication and tumor cell lysis in vitro and, in fact, synergized pharmacologically on Chou-Talalay analysis. In vivo, the combination of the demethylating agents, 5-Aza or decitabine, with rQNestin34.5 significantly prolonged the survivorship of athymic mice harboring intracranial human glioma xenografts over single agent alone.
CONCLUSION: These results, thus, provide further justification for the exploration of demethylating agents when combined with the OV, rQNestin34.5, in preclinical therapeutics and, possibly, clinical trials for malignant glioma.
EXPERIMENTAL DESIGN: We used demethylating drugs [5-azacytidine (5-Aza)], decitabine, or valproic acid (VPA) in both in vitro and in vivo malignant glioma models to determine if they improved the efficacy of rQNestin34.5 therapy.
RESULTS: The use of demethylating agents, such as 5-Aza, improved OV replication and tumor cell lysis in vitro and, in fact, synergized pharmacologically on Chou-Talalay analysis. In vivo, the combination of the demethylating agents, 5-Aza or decitabine, with rQNestin34.5 significantly prolonged the survivorship of athymic mice harboring intracranial human glioma xenografts over single agent alone.
CONCLUSION: These results, thus, provide further justification for the exploration of demethylating agents when combined with the OV, rQNestin34.5, in preclinical therapeutics and, possibly, clinical trials for malignant glioma.
Предметные рубрики MeSH
AnimalsAzacitidineCell Line, TumorCell SurvivalCpG IslandsDNA MethylationDisease Models, AnimalEnhancer Elements, GeneticFemaleGene Expression Regulation, ViralGenetic TherapyGenetic VectorsGliomaHerpesvirus 1, HumanHumansMiceNestinOncolytic VirotherapyOncolytic VirusesPromoter Regions, GeneticViral ProteinsVirus ReplicationXenograft Model Antitumor Assays