Annales Academiae Medicae Stetinensis · 2009
[The role of interleukin-1 gene polymorphism in gastric carcinogenesis associated with Helicobacter pylori infection].
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Brzeźniakiewicz Katarzyna
Аннотация
INTRODUCTION: The search for risk factors of gastric cancer has a long history: H. pylori infection is one of them. Unfortunately, there is no reliable method for the identification of patients at significant risk of gastric cancer among those infected.
AIM: To disclose the role of IL-1B gene polymorphism in gastric cancer by comparing IL-1B genotypes of patients with different, almost mutually exclusive clinical courses.
SUBJECTS: We initially enrolled 273 patients and continued with 259 patients who tested positive for antibodies against H. pylori, including 121 patients with gastric cancer, 119 patients with duodenal ulcer, and 19 patients with asymptomatic gastritis. The study group included 19 families (48 patients) with 14 cases of gastric cancer, 15 cases of duodenal ulcer, and 19 asymptomatic individuals.
MATERIAL AND METHODS: The IL-1B gene was sequenced at position -511 and -31 using primer extension and mass spectrometry. Intron 2 of the IL-1RN gene was studied using PCR and agarose gel electrophoresis. The odds ratio (OR) with 95% confidence intervals was computed using logistic regression.
RESULTS: No association was found between IL-1B gene polymorphism and clinical presentation in 19 families. The differences between patients with gastric cancer and duodenal ulcer applied only to the C/T variant at position -511 of the IL-1B gene after adjustment for family history. Patients with the IL-1B-511 CT genotype and negative family history of gastric cancer demonstrated a greater risk of gastric cancer compared with patients with positive family history.
CONCLUSION: Our results suggest a complex association between IL-1B gene polymorphism, H. pylori infection, and gastric secretion.
AIM: To disclose the role of IL-1B gene polymorphism in gastric cancer by comparing IL-1B genotypes of patients with different, almost mutually exclusive clinical courses.
SUBJECTS: We initially enrolled 273 patients and continued with 259 patients who tested positive for antibodies against H. pylori, including 121 patients with gastric cancer, 119 patients with duodenal ulcer, and 19 patients with asymptomatic gastritis. The study group included 19 families (48 patients) with 14 cases of gastric cancer, 15 cases of duodenal ulcer, and 19 asymptomatic individuals.
MATERIAL AND METHODS: The IL-1B gene was sequenced at position -511 and -31 using primer extension and mass spectrometry. Intron 2 of the IL-1RN gene was studied using PCR and agarose gel electrophoresis. The odds ratio (OR) with 95% confidence intervals was computed using logistic regression.
RESULTS: No association was found between IL-1B gene polymorphism and clinical presentation in 19 families. The differences between patients with gastric cancer and duodenal ulcer applied only to the C/T variant at position -511 of the IL-1B gene after adjustment for family history. Patients with the IL-1B-511 CT genotype and negative family history of gastric cancer demonstrated a greater risk of gastric cancer compared with patients with positive family history.
CONCLUSION: Our results suggest a complex association between IL-1B gene polymorphism, H. pylori infection, and gastric secretion.