Archives of oral biology · 2009
Influence of ADAM28 on biological characteristics of human dental follicle cells.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhao Zheng, Liu Hongchen, Jin Yan, Lingling E
Аннотация
OBJECTIVES: The aim of this study was to investigate the effects of a disintegrin and metalloproteinase 28 (ADAM28) on the biological characteristics of human dental follicle cells (HDFCs) and possible action mechanism.
METHODS: Eukaryotic expression plasmid containing ADAM28 coding region and ADAM28 antisense oligodeoxynucleotides (AS-ODN) with FITC labelling were constructed and synthesised by gene clone and recombination. Then we respectively transfected them into HDFCs by Lipofectamine 2000 system and detected their effects on proliferation, differentiation and apoptosis of HDFCs by MTT assay, cell cycle detection, ALP activity and Annexin V-FITC/PI analysis. Finally we observed the effects of ADAM28 AS-ODN on HDFCs expressing extracellular matrix (ECM) proteins by immunocytochemical staining.
RESULTS: ADAM28 eukaryotic plasmid was constructed and identified successfully, and could be correctly translated and expressed in HDFCs, furthermore overexpression of ADAM28 promoted the HDFCs proliferation and inhibited specific differentiation of HDFCs, while inhibition of ADAM28 exerted the opposite effects and induced apoptosis. Moreover ADAM28 could significantly inhibit the secretion of OPN and type III collagen of HDFCs.
CONCLUSIONS: ADAM28 might actively participate in the network regulation which associates HDFCs proliferation, differentiation, apoptosis with matrix mineralisation during tooth development by interacting with multiple signal molecules.
METHODS: Eukaryotic expression plasmid containing ADAM28 coding region and ADAM28 antisense oligodeoxynucleotides (AS-ODN) with FITC labelling were constructed and synthesised by gene clone and recombination. Then we respectively transfected them into HDFCs by Lipofectamine 2000 system and detected their effects on proliferation, differentiation and apoptosis of HDFCs by MTT assay, cell cycle detection, ALP activity and Annexin V-FITC/PI analysis. Finally we observed the effects of ADAM28 AS-ODN on HDFCs expressing extracellular matrix (ECM) proteins by immunocytochemical staining.
RESULTS: ADAM28 eukaryotic plasmid was constructed and identified successfully, and could be correctly translated and expressed in HDFCs, furthermore overexpression of ADAM28 promoted the HDFCs proliferation and inhibited specific differentiation of HDFCs, while inhibition of ADAM28 exerted the opposite effects and induced apoptosis. Moreover ADAM28 could significantly inhibit the secretion of OPN and type III collagen of HDFCs.
CONCLUSIONS: ADAM28 might actively participate in the network regulation which associates HDFCs proliferation, differentiation, apoptosis with matrix mineralisation during tooth development by interacting with multiple signal molecules.
Предметные рубрики MeSH
ADAM ProteinsAlkaline PhosphataseAnnexin A5ApoptosisCell CycleCell DifferentiationCell ProliferationChildCollagen Type IIIColoring AgentsDental SacDisintegrinsEnzyme InhibitorsExtracellular Matrix ProteinsFluorescein-5-isothiocyanateFluorescent DyesHumansIntegrin-Binding SialoproteinMembrane GlycoproteinsOligodeoxyribonucleotides, AntisenseOsteopontinPhosphoproteinsPlasmidsSialoglycoproteinsTetrazolium SaltsThiazolesTransfectionDentin Sialophosphoprotein