Anticancer research · 2003
The colon mitosis-inhibitor pyroglutamyl-histidyl-glycine alters expression of immediate-early cancer-related genes in HT-29 cells.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Reichelt Wenche H, Yndestad Arne, Wright Marianne S, Elgjo Kjell, Haug Terje, Reichelt Karl L
Аннотация
BACKGROUND: The intestinal mitosis-inhibiting peptide pyroglu-His-GlyOH (pEHG), inhibits normal intestinal epithelial cells and the human colon adenocarcinoma cell line HT-29 and increases the expression of c-fos (1). In this study, we investigated the mechanisms of the growth-inhibiting effects of pEHG.
MATERIALS AND METHODS: cDNA expression array was hybridized with cDNA from HT-29 cells exposed to pEHG or control. The results were confirmed with Northern blot or real-time PCR.
RESULTS: pEHG(1 nM) provoked a significant increase in the expression of the early growth response protein 1 (egr-1) after an incubation of 20 minutes, while c-fos was confirmed up-regulated by the same treatment. We further studied the expression of fosB, c-jun and junB, in the AP-1 complex. fosB was up-regulated 20-fold, but only minor effects on jun variants were observed.
CONCLUSION: pEHG stimulates the gene expression of some immediate-early transcription factors involved in cell proliferation.
MATERIALS AND METHODS: cDNA expression array was hybridized with cDNA from HT-29 cells exposed to pEHG or control. The results were confirmed with Northern blot or real-time PCR.
RESULTS: pEHG(1 nM) provoked a significant increase in the expression of the early growth response protein 1 (egr-1) after an incubation of 20 minutes, while c-fos was confirmed up-regulated by the same treatment. We further studied the expression of fosB, c-jun and junB, in the AP-1 complex. fosB was up-regulated 20-fold, but only minor effects on jun variants were observed.
CONCLUSION: pEHG stimulates the gene expression of some immediate-early transcription factors involved in cell proliferation.
Предметные рубрики MeSH
Bacterial ProteinsCarcinomaColonic NeoplasmsDNA, ComplementaryDNA-Binding ProteinsEarly Growth Response Protein 1Gene Expression ProfilingGene Expression Regulation, NeoplasticGenes, Immediate-EarlyGenes, fosGenes, junGrowth InhibitorsHumansImmediate-Early ProteinsIntestinal MucosaMitosisNeoplasm ProteinsOligonucleotide Array Sequence AnalysisOligopeptidesProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junPyrrolidonecarboxylic AcidTranscription Factor AP-1Transcription Factors