Anticancer research · 2000
In vivo growth inhibition of human colon carcinoma cells (HT-29) by all-trans-retinoic acid, difluoromethylornithine, and colon mitosis inhibitor, individually and in combination.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Paulsen J E, Lützow-Holm C
Аннотация
BACKGROUND: An important strategy in experimental chemotherapy of cancer is to combine agents with different mechanisms of action in order to achieve enhanced effect by synergism. We studied the combination of all-trans-retinoic acid (RA), difluoromethylornithine (DFMO) and the tripeptide colon mitosis inhibitor (CMI) on the in vivo growth of human colon carcinoma cells (HT-29) in athymic mice.
MATERIALS AND METHODS: DFMO was given as 2% in the drinking water. RA (400 nmol/animal) and CMI (1 pmol/animal) were administered i.p. daily.
RESULTS: RA, DFMO or CMI given alone, reduced the tumour growth by 40-60% (P < 0.001). When DFMO was given as a standard therapy, significant synergism was achieved with RA (P = 0.008). In contrast, CMI acted antagonistically with DFMO (P = 0.013). DFMO + RA + CMI was not more effective than DFMO + RA. Without DFMO administration, CMI achieved synergism with RA (P < 0.001).
CONCLUSIONS: Synergism was shown between RA and DFMO, and RA and CMI, but not between CMI and DFMO.
MATERIALS AND METHODS: DFMO was given as 2% in the drinking water. RA (400 nmol/animal) and CMI (1 pmol/animal) were administered i.p. daily.
RESULTS: RA, DFMO or CMI given alone, reduced the tumour growth by 40-60% (P < 0.001). When DFMO was given as a standard therapy, significant synergism was achieved with RA (P = 0.008). In contrast, CMI acted antagonistically with DFMO (P = 0.013). DFMO + RA + CMI was not more effective than DFMO + RA. Without DFMO administration, CMI achieved synergism with RA (P < 0.001).
CONCLUSIONS: Synergism was shown between RA and DFMO, and RA and CMI, but not between CMI and DFMO.