Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2015
Apodemus agrarius is a potential natural host of severe fever with thrombocytopenia syndrome (SFTS)-causing novel bunyavirus.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Ni Hongxia, Yang Fan, Li Yongdong, Liu Wenbin, Jiao Suli, Li Zixiong, Yi Bo, Chen Yi, Hou Xiaomei, Hu Fengjiao, Ding Yibo, Bian Guolin, Du Yan, Xu Guozhang, Cao Guangwen
Аннотация
BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is a tick-borne, novel bunyavirus-caused emerging infectious disease. It becomes a threat to public health due to its high fatality rate.
OBJECTIVE: To clarify the epidemiological characteristics of SFTS and natural host(s) of SFTS-causing virus (SFTSV) in East China.
STUDY DESIGN: Serum antibody against SFTSV in 496 healthy villagers was examined by ELISA. SFTSV in acute sera of SFTS cases and lung tissues of house and field mouse/rats were identified using quantitative RT-PCR, cell culture, and sequencing.
RESULTS: A total of 22 laboratory-confirmed SFTS cases were diagnosed between 2012 and 2014, of which 5 (22.7%) reported a history of tick-bites. The seroprevalance of SFTSV antibody was 10.5% in healthy villagers. SFTSV genomic RNAs were identified in 2 of 8 Apodemus agrarius but not in Rattus norvegicus (n=40) and Rattus losea (n=4). The 3 segments of SFTSV from 11 SFTS cases and 2 A. agrarius were successfully sequenced. Phylogenetic analyses indicated that at least 3 different SFTSV strains (inland-type, Ningbo-native-type, and their reassortant-type) were present in Ningbo. The 3 segments of the 2 SFTSV isolates from A. agrarius shared great sequence homologies to those isolated from the patients living in nearby villages.
CONCLUSION: The present study indicated that A. agrarius might be a natural host of SFTSV in East China.
OBJECTIVE: To clarify the epidemiological characteristics of SFTS and natural host(s) of SFTS-causing virus (SFTSV) in East China.
STUDY DESIGN: Serum antibody against SFTSV in 496 healthy villagers was examined by ELISA. SFTSV in acute sera of SFTS cases and lung tissues of house and field mouse/rats were identified using quantitative RT-PCR, cell culture, and sequencing.
RESULTS: A total of 22 laboratory-confirmed SFTS cases were diagnosed between 2012 and 2014, of which 5 (22.7%) reported a history of tick-bites. The seroprevalance of SFTSV antibody was 10.5% in healthy villagers. SFTSV genomic RNAs were identified in 2 of 8 Apodemus agrarius but not in Rattus norvegicus (n=40) and Rattus losea (n=4). The 3 segments of SFTSV from 11 SFTS cases and 2 A. agrarius were successfully sequenced. Phylogenetic analyses indicated that at least 3 different SFTSV strains (inland-type, Ningbo-native-type, and their reassortant-type) were present in Ningbo. The 3 segments of the 2 SFTSV isolates from A. agrarius shared great sequence homologies to those isolated from the patients living in nearby villages.
CONCLUSION: The present study indicated that A. agrarius might be a natural host of SFTSV in East China.
Предметные рубрики MeSH
AdolescentAdultAgedAnimalsAntibodies, ViralBunyaviridae InfectionsChildChild, PreschoolChinaDisease ReservoirsEnzyme-Linked Immunosorbent AssayFemaleHumansInfantLungMaleMiddle AgedMurinaePhlebovirusReal-Time Polymerase Chain ReactionSequence Analysis, DNASeroepidemiologic StudiesVirus CultivationYoung Adult