Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association · 2004
Cartilage debris increases the expression of chondrodestructive tumor necrosis factor-alpha by articular chondrocytes.
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Cameron-Donaldson Michelle, Holland Christoph, Hungerford David S, Frondoza Carmelita G
Аннотация
PURPOSE: To determine if cartilage particles increased the expression of TNF-alpha by articular chondrocytes.
TYPE OF STUDY: In vitro experiment.
METHODS: Articular chondrocytes were obtained from patients undergoing primary total knee arthroplasty for osteoarthritis (n = 3) and from patients undergoing below-knee amputation for peripheral vascular disease (n = 3). Chondrocytes were then incubated with and without cartilage particles at a concentration of 5 microg/10(5) cells for 24 hours. TNF-alpha levels were then determined using reverse transcription polymerase chain reaction.
RESULTS: Both normal and osteoarthritic chondrocytes had low baseline expression of TNF-alpha under standard cell culture conditions. Expression was markedly increased in response to incubation with cartilage particles, and was statistically significant.
CONCLUSIONS: Cartilage debris in the traumatized and osteoarthritic joint may increase the concentration of TNF-alpha in the joint, contributing to joint symptoms and cartilage destruction. Arthroscopic debridement and lavage may improve symptoms by washing these harmful components from the joint.
LEVEL OF EVIDENCE: Level IV.
TYPE OF STUDY: In vitro experiment.
METHODS: Articular chondrocytes were obtained from patients undergoing primary total knee arthroplasty for osteoarthritis (n = 3) and from patients undergoing below-knee amputation for peripheral vascular disease (n = 3). Chondrocytes were then incubated with and without cartilage particles at a concentration of 5 microg/10(5) cells for 24 hours. TNF-alpha levels were then determined using reverse transcription polymerase chain reaction.
RESULTS: Both normal and osteoarthritic chondrocytes had low baseline expression of TNF-alpha under standard cell culture conditions. Expression was markedly increased in response to incubation with cartilage particles, and was statistically significant.
CONCLUSIONS: Cartilage debris in the traumatized and osteoarthritic joint may increase the concentration of TNF-alpha in the joint, contributing to joint symptoms and cartilage destruction. Arthroscopic debridement and lavage may improve symptoms by washing these harmful components from the joint.
LEVEL OF EVIDENCE: Level IV.