Protective Effect of Compound Cotinus coggygria Oral Liquid on Mice Acute Liver Injury Induced by D-Galactosamine.

OBJECTIVE

We aim to investigate the protective effects of compound Cotinus coggygria oral liquid on acute liver injury of mice induced by D-galactosamine (GalN).

METHODS

Acute liver injury mouse model was established by D-GalN. A total of 72 mice were randomly divided into the blank group, model group constructed by D-GalN, Huganning tablet-treated model group (1.0125 g/kg, as the positive control group), and large (30 ml/kg), medium (15 ml/kg) and small (7.5 ml/kg) doses of compound Cotinus coggygria oral liquid-treated model groups. SPSS 17.0 was used for statistical analysis.

RESULTS

Compared to the blank group, the model group demonstrated alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mice serum and liver homogenate was significantly increased (P<0.01), alteration of liver tissue structure and obvious hepatic cytolysis necrosis, indicating successfully establishement of acute liver injury model. Compared with the model group, the liver index is significantly decreased in large and medium doses of compound Cotinus coggygria oral liquid treated groups (P<0.05), and the effects of large doses of compound Cotinus coggygria are more significant (P<0.01). Furthermore, different doses of compound Cotinus coggygria oral liquid can significantly reduce the activity of mice serum ALT, AST (P<0.01). Large and medium doses of compound Cotinus coggygria oral liquid significantly reduce the activities of ALT (P<0.01) and AST in liver homogenate (P<0.05), and also improve the structure of liver tissues, decreasing the amount of hepatic necrosis, which is associated with liver cell regeneration.

CONCLUSION

Compound Cotinus coggygria oral liquid has protective effects on mice acute liver injury induced by D-GalN.