Journal of clinical periodontology · 2026
Spatiotemporally Responsive BEC Nanoplatform Targets Neutrophils to Reprogram Immune Crosstalk and Alleviate Periodontal Inflamm-Ageing in Periodontitis.
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Luo Jiaxin, Chen Jingxia, Ren Sicong, Li Mucong, Zhai Shaobo, Chen Sheng, Feng Jian, Wang Zhibo, Wang Hanchi, Zhou Yanmin
Аннотация
AIM: To investigate the underlying mechanism of immune cell crosstalk in periodontal inflammatory ageing and to explore potential pharmaceutical interventions for safely reversing this process.
MATERIALS AND METHODS: Bovine serum albumin-epigallocatechin gallate-copper nanoparticles (BEC NPs) were synthesised by coating a bovine serum albumin membrane onto an epigallocatechin gallate-copper-phenolic network. BEC NPs' regulatory impacts on the fate of neutrophils and macrophages were evaluated through immunofluorescence staining, PCR, RNA sequencing and Western blot analysis. Their anti-senescence effects on gingival fibroblasts were assessed using cell migration assays and SA-β-gal staining, while an experimental periodontitis rat model was established to validate the in vitro findings.
RESULTS: Accumulating evidence indicated that pH-responsive nanoparticles alleviated periodontal inflamm-ageing through modulation of neutrophil-macrophage crosstalk. Functional analyses revealed that BEC NPs suppressed neutrophil extracellular trap formation via dual mechanisms: reactive oxygen species scavenging to sustain mitochondrial integrity, and actin cytoskeleton stabilisation to inhibit nuclear translocation of neutrophil elastase and myeloperoxidase. In vivo assessment demonstrated that BEC NPs exhibited a favourable biosafety profile and significant therapeutic efficacy in suppressing the progression of periodontal inflammation.
CONCLUSION: This study presents a smart nanosystem-based 'endogenous homeostasis reconstruction' strategy, offering programmable, early-stage intervention for periodontal inflamm-ageing with considerable clinical translation prospects.
MATERIALS AND METHODS: Bovine serum albumin-epigallocatechin gallate-copper nanoparticles (BEC NPs) were synthesised by coating a bovine serum albumin membrane onto an epigallocatechin gallate-copper-phenolic network. BEC NPs' regulatory impacts on the fate of neutrophils and macrophages were evaluated through immunofluorescence staining, PCR, RNA sequencing and Western blot analysis. Their anti-senescence effects on gingival fibroblasts were assessed using cell migration assays and SA-β-gal staining, while an experimental periodontitis rat model was established to validate the in vitro findings.
RESULTS: Accumulating evidence indicated that pH-responsive nanoparticles alleviated periodontal inflamm-ageing through modulation of neutrophil-macrophage crosstalk. Functional analyses revealed that BEC NPs suppressed neutrophil extracellular trap formation via dual mechanisms: reactive oxygen species scavenging to sustain mitochondrial integrity, and actin cytoskeleton stabilisation to inhibit nuclear translocation of neutrophil elastase and myeloperoxidase. In vivo assessment demonstrated that BEC NPs exhibited a favourable biosafety profile and significant therapeutic efficacy in suppressing the progression of periodontal inflammation.
CONCLUSION: This study presents a smart nanosystem-based 'endogenous homeostasis reconstruction' strategy, offering programmable, early-stage intervention for periodontal inflamm-ageing with considerable clinical translation prospects.