Clinical & experimental ophthalmology · 2018
Investigation of corneal endothelial changes post selective laser trabeculoplasty.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Leahy Kate E, Madigan Michele C, Sarris Maria, Watson Stephanie L, McCluskey Peter, White Andrew Jr
Аннотация
IMPORTANCE: Transient corneal endothelial changes are routinely noted on slit-lamp examination immediately following selective laser trabeculoplasty (SLT).
BACKGROUND: To determine the mechanism of transient corneal endothelial changes observed following SLT.
DESIGN: University laboratory-based observational study.
SAMPLES: Ten corneas from six human cadaveric donors.
METHODS: Corneas were treated with SLT, direct laser or peroxide, or used as controls. Haematoxylin and eosin staining and immunolabelling for zonula occludens-1 (ZO-1) and beta-catenin were performed.
MAIN OUTCOME MEASURES: Histological appearance; ZO-1 and beta-catenin immunostaining.
RESULTS: There were no differences in histological features between SLT-treated and control corneas. Corneas treated with SLT or peroxide showed reduced and less regular ZO-1 immunofluorescence along cell membranes compared with ZO-1 expression in controls. These changes were generalized across the endothelium. There was no effect on the ZO-1 immunostaining after direct laser. There was no difference in beta-catenin immunostaining patterns between control, SLT and peroxide-treated corneas.
CONCLUSIONS AND RELEVANCE: Altered ZO-1 immunostaining may represent disassembly of tight junctions between corneal endothelial cells. The similarity of our findings between SLT-treated and peroxide-treated corneas suggests that both conditions trigger changes at the level of endothelial tight junctions, perhaps triggered by liberation of free radicals as previously proposed.
BACKGROUND: To determine the mechanism of transient corneal endothelial changes observed following SLT.
DESIGN: University laboratory-based observational study.
SAMPLES: Ten corneas from six human cadaveric donors.
METHODS: Corneas were treated with SLT, direct laser or peroxide, or used as controls. Haematoxylin and eosin staining and immunolabelling for zonula occludens-1 (ZO-1) and beta-catenin were performed.
MAIN OUTCOME MEASURES: Histological appearance; ZO-1 and beta-catenin immunostaining.
RESULTS: There were no differences in histological features between SLT-treated and control corneas. Corneas treated with SLT or peroxide showed reduced and less regular ZO-1 immunofluorescence along cell membranes compared with ZO-1 expression in controls. These changes were generalized across the endothelium. There was no effect on the ZO-1 immunostaining after direct laser. There was no difference in beta-catenin immunostaining patterns between control, SLT and peroxide-treated corneas.
CONCLUSIONS AND RELEVANCE: Altered ZO-1 immunostaining may represent disassembly of tight junctions between corneal endothelial cells. The similarity of our findings between SLT-treated and peroxide-treated corneas suggests that both conditions trigger changes at the level of endothelial tight junctions, perhaps triggered by liberation of free radicals as previously proposed.