Immunolocalization of B-50 (GAP-43) in intact and lesioned neurohypophysis of adult rats.

The persistence of high levels of B-50 in the adult brain is generally assumed to characterize neuronal systems capable of undergoing some form of plasticity such as axonal sprouting and regeneration. Since adult hypothalamo-neurohypophysial neurons are known to rapidly regenerate after being transected, the present study was undertaken to determine if such a capacity for regeneration could be related to the expression of this protein. Adult rats were killed by intraaortic perfusion of fixative either without lesion or at different delays after a surgical transection of the hypophysial stalk. Electron microscopy and laser scanning confocal microscopy were used to examine the regenerating axons after single or double immunocytochemical labeling of vibratome sections for B-50 and for various neuronal markers characterizing different types of neurohypophysial axons. In intact neurohypophysis, B-50 immunostaining was frequently associated with fibers immunoreactive to GABA or to tyrosine hydroxylase, whereas it was not detected within peptidergic neurohypophysial axons. In the lesioned neurohypophysis, B-50 was again frequently localized within axonal fibers immunoreactive to tyrosine hydroxylase or GABA. On the other hand, B-50 immunostaining was never detected within the numerous vasopressinergic or oxytocinergic axonal sprouts that regenerate all along the median eminence proximal to the lesion. These data indicate that persistence of high levels of B-50 within the neurohypophysis of adult rats is a specific feature of catecholaminergic and/or GABA-ergic axons innervating this region and that, contrasting to other neuronal systems, B-50 is not involved in the remarkable capacity for regeneration exhibited by the vasopressinergic and oxytocinergic neurohypophysial axons.