Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi · 1998
[Changes in the contents of membrane phospholipid and membrane phospholipid asymmetry in glucose-6-phosphate dehydrogenase deficient erythrocyte].
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Zou C, Tian X, Yan H
Аннотация
OBJECTIVE: To study the effect of G6PD deficiency on membrane phospholipid structures.
METHODS: Treatment of intact erythrocyte with phospholipase A2 was used for the membrane phospholipid asymmetry. Membrane phospholipid was determined by high performance liquid chromatography (HPLC).
RESULTS: The contents of phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS) were 5.18 +/- 1.86, 1.51 +/- 0.58 and 5.70 +/- 1.91 mg/mg membrane protein, respectively, in G6PD deficient erythrocytes and were 5.59 +/- 1.16, 2.92 +/- 0.50 and 6.64 +/- 0.92 mg/mg membrane protein, respectively, in normal erythrocytes. In normal erythrocytes, 17.6% +/- 2.5% PE and 57.9% +/- 4.7% PC were present and no PS was found on the outer leaflet of the membrane. While in G6PD deficient ones, 30.2% +/- 5.7% PE, 53.8% +/- 7.4% PC and 27.2% +/- 5.8% PS were present on the outer leaflet.
CONCLUSION: Membrane phospholipid changes in G6PD deficient erythrocytes might be one of the factors causing hemolysis.
METHODS: Treatment of intact erythrocyte with phospholipase A2 was used for the membrane phospholipid asymmetry. Membrane phospholipid was determined by high performance liquid chromatography (HPLC).
RESULTS: The contents of phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS) were 5.18 +/- 1.86, 1.51 +/- 0.58 and 5.70 +/- 1.91 mg/mg membrane protein, respectively, in G6PD deficient erythrocytes and were 5.59 +/- 1.16, 2.92 +/- 0.50 and 6.64 +/- 0.92 mg/mg membrane protein, respectively, in normal erythrocytes. In normal erythrocytes, 17.6% +/- 2.5% PE and 57.9% +/- 4.7% PC were present and no PS was found on the outer leaflet of the membrane. While in G6PD deficient ones, 30.2% +/- 5.7% PE, 53.8% +/- 7.4% PC and 27.2% +/- 5.8% PS were present on the outer leaflet.
CONCLUSION: Membrane phospholipid changes in G6PD deficient erythrocytes might be one of the factors causing hemolysis.