Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases · 2011
[Effect of physicochemical factors on infectivity of Spirometra mansoni plerocercoid].
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Tang Gui-Wen, Cheng Yan
Аннотация
OBJECTIVE: To observe the effect of different physicochemical factors on the infectivity of Spirometra mansoni plerocercoids.
METHODS: The muscle samples with plerocercoids taken from Rana nigromaculata (about 1 cm3 each piece) were treated with different temperature (-20 degrees C, 4 degrees C, 37 degrees C and 56 degrees C) or different concentrations of ethanol (20%, 30%, 40%, 50% and 60%) for 1, 2 or 3 h, or soaked in ginger juice, vinegar (total acid concentration of 4.5%, pH 3.05) or soy sauce (containing 19.3% NaCl) for 3, 6, 12 or 24 h. The muscle with plerocercoids treated with normal saline under 20 degrees C served as control. 30 plerocercoids were used under each condition and fed to 10 mice averagely (3 larvae/mouse). Another 20 plerocercoids with frog meat were comminuted for 3 min then fed to 10 mice. One week later, the mice were sacrificed to collect the parasitic plerocercoids and the number of positive mice and plerocercoids was recorded.
RESULTS: None of the mice fed with plerocercoids treated under -20 degrees C for 2 h was infected. All the mice fed with plerocercoids treated under 56 degrees C for 2 h and 3 h were infected. The percentage of infective plerocercoids was 60% (18/30) and 43% (13/30), respectively, considerably lower than those of the control (90%, 27/30) (P < 0.05). None of the mice fed with plerocercoids soaked in 60% ethanol for 2 h was infected. All the mice fed with plerocercoids soaked in 60% ethanol for 1 h, or in 50% ethanol for 2 h or 3 h were infected. The percentage of infective plerocercoids was 60% (18/30), 57% (17/30), and 50% (15/30), respectively, considerably lower than those of the control (93%, 28/30) (P<0.05). None of the mice fed with plerocercoids soaked in vinegar for 24 h, or soy sauce for 6 h was infected. The infectivity of the plerocercoids treated by ginger juice for 24 h was similar to the control (P>0.05). Among the ten mice fed with comminuted frog meat with plerocercoids, five were infected with 6 plerocercoids.
CONCLUSION: Treatment with -20 degrees C or 60% ethanol for 2 h, soy sauce for 6 h, or vinegar for 24 h can destroy the infectivity of plerocercoids in 1 cm3 frog muscle.
METHODS: The muscle samples with plerocercoids taken from Rana nigromaculata (about 1 cm3 each piece) were treated with different temperature (-20 degrees C, 4 degrees C, 37 degrees C and 56 degrees C) or different concentrations of ethanol (20%, 30%, 40%, 50% and 60%) for 1, 2 or 3 h, or soaked in ginger juice, vinegar (total acid concentration of 4.5%, pH 3.05) or soy sauce (containing 19.3% NaCl) for 3, 6, 12 or 24 h. The muscle with plerocercoids treated with normal saline under 20 degrees C served as control. 30 plerocercoids were used under each condition and fed to 10 mice averagely (3 larvae/mouse). Another 20 plerocercoids with frog meat were comminuted for 3 min then fed to 10 mice. One week later, the mice were sacrificed to collect the parasitic plerocercoids and the number of positive mice and plerocercoids was recorded.
RESULTS: None of the mice fed with plerocercoids treated under -20 degrees C for 2 h was infected. All the mice fed with plerocercoids treated under 56 degrees C for 2 h and 3 h were infected. The percentage of infective plerocercoids was 60% (18/30) and 43% (13/30), respectively, considerably lower than those of the control (90%, 27/30) (P < 0.05). None of the mice fed with plerocercoids soaked in 60% ethanol for 2 h was infected. All the mice fed with plerocercoids soaked in 60% ethanol for 1 h, or in 50% ethanol for 2 h or 3 h were infected. The percentage of infective plerocercoids was 60% (18/30), 57% (17/30), and 50% (15/30), respectively, considerably lower than those of the control (93%, 28/30) (P<0.05). None of the mice fed with plerocercoids soaked in vinegar for 24 h, or soy sauce for 6 h was infected. The infectivity of the plerocercoids treated by ginger juice for 24 h was similar to the control (P>0.05). Among the ten mice fed with comminuted frog meat with plerocercoids, five were infected with 6 plerocercoids.
CONCLUSION: Treatment with -20 degrees C or 60% ethanol for 2 h, soy sauce for 6 h, or vinegar for 24 h can destroy the infectivity of plerocercoids in 1 cm3 frog muscle.