Ocular immunology and inflammation · 2015
devR PCR for the diagnosis of intraocular tuberculosis.
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Kataria Pankaj, Kumar Abiraj, Bansal Reema, Sharma Aman, Gupta Vishali, Gupta Amod, Singh Ramandeep, Sharma Kusum
Аннотация
PURPOSE: To compare the efficacy of devR and MPB64 PCR in the diagnosis of intraocular tuberculosis.
METHODS: Prospective, nonrandomized study. Seventy-five patients were enrolled in 3 groups. Group A had 25 patients with presumed intraocular tubercular uveitis, group B had 25 controls with specific uveitis other than tubercular uveitis, and group C included 25 non-uveitic negative controls. The undiluted vitreous/aqueous samples were collected and subjected to PCR assay for devR and MPB64 gene sequence of Mycobacterium tuberculosis (MTB) to detect sensitivity and specificity.
RESULTS: devR PCR was positive in 16 (64%) out of 25 patients with presumed tubercular uveitis. MPB64 PCR was positive in 18 (72%) out of 25 patients with presumed tubercular uveitis. The sensitivity and specificity of devR were 64 and 100%, respectively. The sensitivity and specificity of MPB64 PCR were 72 and 100%, respectively.
CONCLUSION: devR PCR is not a better tool than MPB64 PCR for diagnosing intraocular tuberculosis.
METHODS: Prospective, nonrandomized study. Seventy-five patients were enrolled in 3 groups. Group A had 25 patients with presumed intraocular tubercular uveitis, group B had 25 controls with specific uveitis other than tubercular uveitis, and group C included 25 non-uveitic negative controls. The undiluted vitreous/aqueous samples were collected and subjected to PCR assay for devR and MPB64 gene sequence of Mycobacterium tuberculosis (MTB) to detect sensitivity and specificity.
RESULTS: devR PCR was positive in 16 (64%) out of 25 patients with presumed tubercular uveitis. MPB64 PCR was positive in 18 (72%) out of 25 patients with presumed tubercular uveitis. The sensitivity and specificity of devR were 64 and 100%, respectively. The sensitivity and specificity of MPB64 PCR were 72 and 100%, respectively.
CONCLUSION: devR PCR is not a better tool than MPB64 PCR for diagnosing intraocular tuberculosis.