Pest management science · 2025
Hyaluronic acid-functionalized nanoarmor enhances the stable colonization ability of Paenibacillus polymyxa JF_P68 and boosts its biological control efficacy against pear anthracnose.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Du Jianfeng, Li Heng, Liu Shenjie, Wu Limin, Liu Yiming, Li Yue, Zhao Xin, Gao Qixiong, Dong Jing, Lei Chen, Sun Fuxin, Tian Xueliang, Wang Chuanjun, Lu Ninghai, Jiao Yang
Аннотация
BACKGROUND: The application of biocontrol agents offers a promising strategy for managing pear anthracnose, yet their efficacy is often limited by unstable colonization on fruit surfaces.
RESULTS: Here, we isolated Paenibacillus polymyxa JF_P68, which exhibits strong inhibitory activity against Colletotrichum fructicola. To enhance its environmental tolerance, we developed a hyaluronic acid (HA)-functionalized nanoarmor using a layer-by-layer coating technique. This nanoarmor significantly improved the JF_P68's resistance to ultraviolet radiation and oxidative stress. In vivo trials demonstrated that nanoarmor-equipped JF_P68 reduced pear anthracnose lesion diameters by 43.5% and maintained higher colonization levels on fruit surfaces. Genome analysis revealed 20 biosynthetic gene clusters, including novel antimicrobial candidates, underpinning its broad-spectrum antifungal activity.
CONCLUSION: The single-cell coating method, characterized by simplicity, scalability, and biocompatibility, significantly enhances the persistence and efficacy of biocontrol agents. This study provides a novel approach to improving the biological control of pear anthracnose. © 2025 Society of Chemical Industry.
RESULTS: Here, we isolated Paenibacillus polymyxa JF_P68, which exhibits strong inhibitory activity against Colletotrichum fructicola. To enhance its environmental tolerance, we developed a hyaluronic acid (HA)-functionalized nanoarmor using a layer-by-layer coating technique. This nanoarmor significantly improved the JF_P68's resistance to ultraviolet radiation and oxidative stress. In vivo trials demonstrated that nanoarmor-equipped JF_P68 reduced pear anthracnose lesion diameters by 43.5% and maintained higher colonization levels on fruit surfaces. Genome analysis revealed 20 biosynthetic gene clusters, including novel antimicrobial candidates, underpinning its broad-spectrum antifungal activity.
CONCLUSION: The single-cell coating method, characterized by simplicity, scalability, and biocompatibility, significantly enhances the persistence and efficacy of biocontrol agents. This study provides a novel approach to improving the biological control of pear anthracnose. © 2025 Society of Chemical Industry.