mBio · 2025
Dual function of Candida auris mannosyltransferase, MNT5, in biofilm community protection from antifungal therapy and the host.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Zarnowski Robert, Horton Mark V, Johnson Chad J, Vang Por Choua, Uram Jeremy, Fernando Liyanage Devthilini Pasasum, Vlach Jiri, Heiss Christian, Azadi Parastoo, Nett Jeniel E, Andes David R
Аннотация
UNLABELLED: Screen of mutants from a mannosyltransferase family identified the importance of MNT5 for C. auris biofilm drug resistance and neutrophil evasion. Biochemical analysis of the mnt5∆ mutant matrix and cell wall identified alterations in the mannan structures. Resistance and matrix for mnt5∆ were restored with delivery of wild-type matrix via extracellular vesicles. Analysis of the mnt5∆ cell wall revealed a reduction in mannan and compensatory increase in cell surface glucan and chitin, suggesting a role for MNT5 in mannan masking of pathogen-associated molecular patterns.
IMPORTANCE: C. auris recalcitrance is linked to biofilm drug resistance and immune evasion. The mannosyltransferase encoded by MNT5 is necessary for both phenotypes and may serve as a useful therapeutic target.
IMPORTANCE: C. auris recalcitrance is linked to biofilm drug resistance and immune evasion. The mannosyltransferase encoded by MNT5 is necessary for both phenotypes and may serve as a useful therapeutic target.