The Journal of infectious diseases · 2017
Elevated Pentraxin-3 Concentrations in Patients With Leprosy: Potential Biomarker of Erythema Nodosum Leprosum.
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Mendes Mayara Abud, de Carvalho Daniel Serra, Amadeu Thaís Porto, Silva Bruno Jorge de Andrade, Prata Rhana Berto da Silva, da Silva Camila Oliveira, Ferreira Helen, Hacker Mariana de Andrea, Nery José Augusto Costa, Pinheiro Roberta Olmo, Sampaio Elizabeth Pereira, Sarno Euzenir Nunes, Schmitz Veronica
Аннотация
BACKGROUND: Leprosy, the leading infectious cause of disability worldwide, remains a major public health challenge in the most severely affected countries despite the sharp decline in new cases in recent years. The search for biomarkers is essential to achieve a better understanding of the molecular and cellular mechanisms underlying the disease.
METHODS: Pentraxin-3 (PTX3) analyses of sera from 87 leprosy patients with or without reactions were conducted via enzyme-linked immunosorbent assay. In situ identification of PTX3 in skin lesion was confirmed by quantitative reverse-transcription polymerase chain reaction, immunohistochemistry, and immunofluorescence assays.
RESULTS: We found that PTX3 serum levels were higher in multibacillary patients when evaluated before the onset of acute erythema nodosum leprosum (ENL) and persistently elevated during reaction. Thalidomide treatment reduced PTX3 in the serum 7 days after starting treatment. In situ analyses have also demonstrated enhancement of PTX3 in ENL lesions and showed that treatment with thalidomide reduced its expression and the prominent neutrophilic infiltrate, a hallmark of the disease.
CONCLUSIONS: In summary, our study provides in vivo evidence that PTX3 is enhanced during ENL but not in reversal reaction and provides a new molecular target in ENL pathogenesis.
METHODS: Pentraxin-3 (PTX3) analyses of sera from 87 leprosy patients with or without reactions were conducted via enzyme-linked immunosorbent assay. In situ identification of PTX3 in skin lesion was confirmed by quantitative reverse-transcription polymerase chain reaction, immunohistochemistry, and immunofluorescence assays.
RESULTS: We found that PTX3 serum levels were higher in multibacillary patients when evaluated before the onset of acute erythema nodosum leprosum (ENL) and persistently elevated during reaction. Thalidomide treatment reduced PTX3 in the serum 7 days after starting treatment. In situ analyses have also demonstrated enhancement of PTX3 in ENL lesions and showed that treatment with thalidomide reduced its expression and the prominent neutrophilic infiltrate, a hallmark of the disease.
CONCLUSIONS: In summary, our study provides in vivo evidence that PTX3 is enhanced during ENL but not in reversal reaction and provides a new molecular target in ENL pathogenesis.
Предметные рубрики MeSH
AdolescentAdultAgedBiomarkersC-Reactive ProteinCase-Control StudiesChildEnzyme-Linked Immunosorbent AssayErythema NodosumFemaleFluorescent Antibody TechniqueHumansImmunohistochemistryLeprostatic AgentsLeprosy, LepromatousMaleMiddle AgedReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSerum Amyloid P-ComponentSkinThalidomideYoung AdultPentraxins