Glycoproteins expression in apical pathologic tissues: clinical incidences.

Indirect immunofluorescence was used for the localization of the primary collagens, fibronectin and laminin. Specimens were extracted from untreated teeth with periapical lesions from patients 20 to 30 years of age. An histological examination enabled the differentiation of granulomas and cysts, and 5 microns sections were used for the indirect immunofluorescence procedure. Antibodies against Type I, Type III, and Type V collagen and for fibronectin and laminin were obtained from glycoproteins of human cells. The antibody against Type IV collagen was prepared from Type IV collagen of beef retina. All the glycoproteins investigated were expressed in apical lesions. The intensity of the immunostaining appeared more positive at the external area compared with the center of the lesion. The type IV collagen was specific for the basement membrane of cysts. The immunofluorescence reactions of fibronectin and of laminin were similar in intensity in both granulomas and cysts.