How can reasonable and repeatable acaricidal tests of natural products against Sarcoptes and Psoroptes mites be carried out?

Sarcoptic and psoroptic mange threaten the health of both animals and humans worldwide and result in enormous socioeconomic loss. Discovery of new lead compounds is a major goal for controlling this kind of disease. However, the methodological shortcomings of acaricidal tests against Sarcoptes and Psoroptes mites for screening active compounds in vitro troubles colleagues and should be given more attention.