Preparation of amine-functionalized polyacrylonitrile-TiO2-chitosan multilayer nanofibers as a potential wound dressing: Characterization and investigation of in vitro cell viability, proliferation and antibacterial study.

Development of a Multi-layered (ML) nanofibers (NFs) scaffold by combining advanced materials to address the diverse needs of wound healing offers a comprehensive solution. In this study, a ML scaffold composed of amine functionalized polyacrylonitrile (AFP) NFs membrane as base layer, TiO2 NPs (T) as middle layer, and chitosan (CS) NFs membrane as contact layer was fabricated sequentially by electrospinning, surface functionalization and electrospraying to promote the wound healing. The multi-layered NFs scaffold (ML AFPT-CS) demonstrated adequate morphology, porosity, surface roughness and hydrophilicity with a water contact angle of 41.94°. The NFs scaffolds were evaluated for in-vitro cellular activity using NIH3T3-E1cells and antibacterial performance. The in-vitro analysis inferred that ML AFPT-CS scaffold in comparison with other study groups exhibited excellent cell viability proliferation and resulted in a spindle shape morphology with cells extending across the ML AFPT-CS scaffold and spreading over the NFs surface. Similarly, the ML AFPT-CS scaffolds were active against all four types of bacterial pathogens (M. luteus, S. flexeneri, S. aureus and K. pneumonia) with a highest inhibition against M. luteus (1.7 mm). The developed ML AFPT-CS scaffold could be promising candidate for advanced wound dressing in future.