Magnetic resonance in medicine · 2026
Repeatability of Metabolic Imaging With Hyperpolarized Pyruvate: Back-to-Back Neuroimaging and Blood Analysis.
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Chen Jun, Lin Sung-Han, Sudderth Jessica, Derner Kelley A, Harrison Crystal E, Zaidi Maheen, Baxter Jeannie D, Liticker Jeff, Erfani Zohreh, Malloy Craig R, Pinho Marco C, DeBerardinis Ralph J, Park Jae Mo
Аннотация
PURPOSE: 13C MRI with hyperpolarized [1-13C]pyruvate enables noninvasive imaging of metabolic pathways. Considering the high dose of pyruvate used in hyperpolarized 13C-pyruvate studies, circulating 13C-pyruvate and the resulting 13C-lactate in plasma may influence subsequent pyruvate metabolism, yet the guidance on consecutive injections does not exist. This study is to characterize blood pyruvate and lactate dynamics following [1-13C]pyruvate injection and to evaluate the repeatability of 13C neuroimaging with consecutive pyruvate injections.
METHODS: Sixteen healthy adults (mean age, 39.0 ± 15.9 years; 8 men) underwent blood sampling or MRI. Eight participants received bolus injections of non-hyperpolarized [1-13C]pyruvate following both overnight fasting and postprandial states. Blood samples were collected at baseline and up to 60 min post-injection to quantify pyruvate and lactate concentrations and 13C fractional enrichments using mass spectrometry. In parallel, eight participants underwent 13C/1H MRI that included two injections of hyperpolarized [1-13C]pyruvate with intervals of 6-55 min for time-resolved measurement of 13C-pyruvate and 13C-lactate in the brain.
RESULTS: Plasma 13C-pyruvate peaked within 30 s and returned to baseline within 5-10 min, while 13C-lactate peaked at 2-3 min. Lactate 13C enrichment was higher in fasting versus fed states, and total lactate concentration in blood was unchanged by pyruvate injection. Brain 13C-lactate/pyruvate showed excellent repeatability (ICC = 0.9842), with all points within the 95% Bland-Altman limits. Voxel-wise Pearson's correlation between injections was 0.72 ± 0.22 and within-subject coefficient of variation was 17.6% ± 4.6%.
CONCLUSION: Consecutive injections of hyperpolarized [1-13C]pyruvate yield repeatable blood and brain metabolic measurements in humans, supporting multi-injection protocols in 13C MRI studies.
METHODS: Sixteen healthy adults (mean age, 39.0 ± 15.9 years; 8 men) underwent blood sampling or MRI. Eight participants received bolus injections of non-hyperpolarized [1-13C]pyruvate following both overnight fasting and postprandial states. Blood samples were collected at baseline and up to 60 min post-injection to quantify pyruvate and lactate concentrations and 13C fractional enrichments using mass spectrometry. In parallel, eight participants underwent 13C/1H MRI that included two injections of hyperpolarized [1-13C]pyruvate with intervals of 6-55 min for time-resolved measurement of 13C-pyruvate and 13C-lactate in the brain.
RESULTS: Plasma 13C-pyruvate peaked within 30 s and returned to baseline within 5-10 min, while 13C-lactate peaked at 2-3 min. Lactate 13C enrichment was higher in fasting versus fed states, and total lactate concentration in blood was unchanged by pyruvate injection. Brain 13C-lactate/pyruvate showed excellent repeatability (ICC = 0.9842), with all points within the 95% Bland-Altman limits. Voxel-wise Pearson's correlation between injections was 0.72 ± 0.22 and within-subject coefficient of variation was 17.6% ± 4.6%.
CONCLUSION: Consecutive injections of hyperpolarized [1-13C]pyruvate yield repeatable blood and brain metabolic measurements in humans, supporting multi-injection protocols in 13C MRI studies.