Archives of virology · 2023
A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic.
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Ramos Natalia, Panzera Yanina, Frabasile Sandra, Tomás Gonzalo, Calleros Lucía, Marandino Ana, Goñi Natalia, Techera Claudia, Grecco Sofía, Fuques Eddie, Coppola Leticia, Ramas Viviana, Morel Maria Noelia, Mogdasy Cristina, Chiparelli Héctor, Arbiza Juan, Pérez Ruben, Delfraro Adriana
Аннотация
A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Montevideo, Uruguay, was analyzed. The results revealed the cocirculation of SARS-CoV-2 with human rhinovirus (hRV) A, B and C, human respiratory syncytial virus (hRSV) B, influenza A virus, and metapneumovirus B1. SARS-CoV-2 coinfections with hRV or hRSV B and influenza A virus coinfections with hRV C were identified in adults and/or children. This methodology combines the benefits of multiplex genomic amplification with the sensitivity and information provided by NGS. An advantage is that additional viral targets can be incorporated, making it a helpful tool to investigate the cocirculation and coinfections of respiratory viruses in pandemic and post-pandemic contexts.