Cell · 2016
Multiple Origins of Virus Persistence during Natural Control of HIV Infection.
СтатьяПоддержка NIH: внешняя программаResearch Support, N.I.H., IntramuralПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Boritz Eli A, Darko Samuel, Swaszek Luke, Wolf Gideon, Wells David, Wu Xiaolin, Henry Amy R, Laboune Farida, Hu Jianfei, Ambrozak David, Hughes Marybeth S, Hoh Rebecca, Casazza Joseph P, Vostal Alexander, Bunis Daniel, Nganou-Makamdop Krystelle, Lee James S, Migueles Stephen A, Koup Richard A, Connors Mark, Moir Susan, Schacker Timothy, Maldarelli Frank, Hughes Stephen H, Deeks Steven G, Douek Daniel C
Аннотация
Targeted HIV cure strategies require definition of the mechanisms that maintain the virus. Here, we tracked HIV replication and the persistence of infected CD4 T cells in individuals with natural virologic control by sequencing viruses, T cell receptor genes, HIV integration sites, and cellular transcriptomes. Our results revealed three mechanisms of HIV persistence operating within distinct anatomic and functional compartments. In lymph node, we detected viruses with genetic and transcriptional attributes of active replication in both T follicular helper (TFH) cells and non-TFH memory cells. In blood, we detected inducible proviruses of archival origin among highly differentiated, clonally expanded cells. Linking the lymph node and blood was a small population of circulating cells harboring inducible proviruses of recent origin. Thus, HIV replication in lymphoid tissue, clonal expansion of infected cells, and recirculation of recently infected cells act together to maintain the virus in HIV controllers despite effective antiviral immunity.