Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Strains Isolated from Diseased Captive Giant Pandas (Ailuropoda melanoleuca) in China.

Objectives

To characterize the antimicrobial resistance and virulence of pathogenic Escherichia coli isolated from diseased captive giant pandas.

Methods

Antimicrobial susceptibility and minimum inhibitory concentration (MIC) were determined by the broth dilution method. Whole-genome sequencing was used to characterize the phylogeny, serotype, virulence, resistome, plasmids, and genetic structures of the cefotaxime (CTX)-M genes.

Results

Four extended-spectrum beta-lactamase (ESBL)-producing E. coli strains were identified and the MICs against 11 antibiotics in vitro were determined. All ESBL-producing E. coli strains were resistant to more than eight antibiotics and carried the blaCTX-M-55 or blaCTX-M-105 gene in different sizes of replicon-type plasmids (pAMSH1-IncHI2, 257 kb; pAMPD2-IncFII, 89 kb; pAMPD02-IncFIB, 129 kb; and pAMSC4-IncN, 47 kb). Distinct insertional sequences and transposases were identified up-/downstream of blaCTX-Ms, including IS26, ISEcp1, ISKpn72, IS903B, and Tn2. These strains also possessed at least three virulence genes of pathogenic E. coli and originated in four different evolutionary branches. One strain carried the complete locus of the enterocyte effacement pathogenicity island, but lacked the virulence genes stx and bfpA, indicating atypical enteropathogenic E. coli, whereas the other strains were considered to be extraintestinal pathogenic E. coli.

Conclusions

The emergence of ESBL-producing pathogenic E. coli strains from diseased captive giant pandas warrants greater attention. The findings of this study will help to prevent the spread of these strains among captive giant pandas as well as from wild animals to humans.